Cyclohexane, oxidized, non-acidic by-products, distn. residues

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Unsuitable test system (no characterization of the test substance, no specific substance can be anticipated for the effects due to presence of multiple constituents test solution)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1962

Report date:

1962

Materials and methods

Principles of method if other than guideline:

BASF Test: This test (also called inhalation hazard test) was performed in principle as described in OECD Guideline 403. It demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of a test substance at the temperature chosen for vapor generation (20 °C and 1002.6 hPa). One group of 3 rats per sex were exposed sequentially to the vapors, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for different time periods. No analytical determination of the atmosphere concentrations was performed. The nominal concentration was calculated as quotient of the amount of test substance weight loss during the exposure, and the amount of air used during the exposure. Group-wise documentation of clinical signs was performed over the 8-day study period. Body weight of groups was determined before the start of the study and at the end of the observation period in the surviving animals. The clinical signs and findings were reported in summarized form. The study allows for an estimate of the length of time required to cause severe toxic effects resulting from exposure to an atmosphere saturated with volatile components of the test substance. The test with no mortalities was repeated.

GLP compliance:

no

Test type:

other: Inhalation hazard test

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Cyclohexane, oxidized, non-acidic by-products, distn. residues

EC Number:

271-816-4

EC Name:

Cyclohexane, oxidized, non-acidic by-products, distn. residues

Cas Number:

68609-04-1

IUPAC Name:

(1s,4s)-cyclohexane-1,4-diol (cyclohexyloxy)cyclohexane 2-butylcyclohexan-1-one 8-oxatricyclo[7.4.0.0^{2,7}]trideca-1(9),2(7)-diene cyclohexane cyclohexane-1,3-diol cyclohexanol phenol hydrate

Details on test material:

- Name of test material (as cited in study report): Liquid Anon-destillation residue
- condensation products of cyclohexanol and cyclohexanon of unknown chemical structure. Ash-content approx. 2% (determined as sulfate-ash).

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Mean weight at study initiation:
Main test 195 g
Repeat test 190 g

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

0.54 mg/l (calculated by substance loss)

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 8 days
- Frequency of observations and weighing: mortality and clinical signs of toxicity were observed several times on the application day and thereafter once each working day, body weights were only recorded at the beginning of the study.
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 0.54 mg/L air

Exp. duration:

8 h

Mortality:

no mortalities were observed

Clinical signs:

other: no signs during or after exposure were observed

Gross pathology:

no abnormalities were detected