Cuprate(6-), [2-[[[[3-[[4-chloro-6-[[4-[[4-chloro-6-[(3-sulfophenyl)amino]-1,3,5-triazin-2-yl]amino]-2,5-disulfophenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulfophenyl]azo]phenylmethyl]azo]-5-sulfobenzoato(8-)]-, pentasodium hydrogen, (SP-4-3)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Japanese Ministry of Trade and Industry Test Protocol for Testing of New Chemical Substances

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: preparation of fresh dye solutions with test water (Brixham tap water)
- Controls: negative
- Evidence of undissolved material: highly coloured test solutions, undissolved material is not mentioned

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Orange Medaka / Japanese Rice Fish
- Length at study initiation (length definition, mean, range and SD): mean = 25 mm
- Weight at study initiation (mean and range, SD): mean = 0.24 g

ACCLIMATION
- Acclimation period: 7 d
- Acclimation conditions (same as test or not): same

FEEDING DURING TEST
- Frequency: no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

60 mg/L (CaCO3)

Test temperature:

25°C

pH:

7.4

Dissolved oxygen:

>80% saturation

Nominal and measured concentrations:

Nominal: 0 (control), 1000, 2500, 5000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 10 L volume
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Brixham tap water
- Chlorine: not detectable
- Culture medium different from test medium: no

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 24 and 48 h

Reference substance (positive control):

not required

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0%
- Survival rate (24 h): control: 100%, 1000 mg/L: 100%, 2500 mg/L: 70%, 5000 mg/L: 10%
- Survival rate (48 h): control: 100%, 1000 mg/L: 100%, 2500 mg/L: 20%, 5000 mg/L: 0%

Validity criteria fulfilled:

yes

Conclusions:

The 48-h LC50 for Oryzias latipes was determined at 1800 mg/L based on nominal concentrations.

Description of key information

The 48-h LC50 for Oryzias latipes was determined at 1800 mg/L based on nominal concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 800 mg/L