Cuprate(6-), [2-[[[[3-[[4-chloro-6-[[4-[[4-chloro-6-[(3-sulfophenyl)amino]-1,3,5-triazin-2-yl]amino]-2,5-disulfophenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulfophenyl]azo]phenylmethyl]azo]-5-sulfobenzoato(8-)]-, pentasodium hydrogen, (SP-4-3)-

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Japanese Ministry of Trade and Industry Test Protocol for Testing of New Chemical Substances

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 D (Bioaccumulation: Static Fish Test)

GLP compliance:

no

Radiolabelling:

no

Details on sampling:

Water samples from the exit to the test vessels were taken at least twice weekly throughout the test. The absorption at 620 nm and 800 nm was measured.

Carp were taken from each of the two test and control vessels at weekly intervals throughout the test, and stored in ''polythene" bags in the deep freeze until the end of the test period. Before analysis the fish were allowed to thaw and weighed. They were then dried at 105°C for 48 hours and re-weighed. Each fish was placed in a quartz/silica flask and 20 mL of "Aristar" nitric acid added. The top of the flask was covered with a watch glass, and the mixture placed on a 130°C hot plate for 24 hours . It was then filtered through a Whatman GF-B glass fibre filter and made up to 25 mL with Q.D.W. These solutions were anlysed for copper using the atomic absorption spectrometer, with deuterium lamp correction for non-atomic absorption.

Vehicle:

not specified

Test organisms (species):

Cyprinus carpio

Details on test organisms:

The fish used were Mirror Carp (Cyprinus carpio) which prior to the test were acclimatised to the test conditions for a period of 7 days. The fish were randomly selected from stock fish which had all been in good health for a period of at least two weeks prior to the acclimatisation period.

Route of exposure:

aqueous

Justification for method:

aqueous exposure method used for following reason: water soluble substance with low log Kow

Test type:

static

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Reference substance (positive control):

no

Details on estimation of bioconcentration:

BASIS INFORMATION
- Measured/calculated logPow: < -6.6

Key result

Conc. / dose:

2.34 mg/L

Temp.:

23.3 °C

pH:

7.8

Type:

BCF

Value:

6 L/kg

Basis:

whole body d.w.

Calculation basis:

steady state

Key result

Conc. / dose:

22.66 mg/L

Temp.:

23.3

pH:

7.8

Type:

BCF

Value:

3 L/kg

Basis:

whole body d.w.

Calculation basis:

steady state

Details on results:

- Mortality of test organisms: no mortality observed
- Behavioural abnormalities: none
- Other biological observations: none
- Organ specific bioaccumulation:
- Bound residues forming a plateau:
- Mortality and/or behavioural abnormalities of control: none observed
- Loss of test substance during test period: average measured concentrations = 2.34 mg/L (2.5 mg/L nominal, recovery 93.6%), 22.66 mg/L (25.0 mg/L nominal, recovery 90.7%)

Validity criteria fulfilled:

not specified

Conclusions:

The 8 wk-BCF in Cyprinus carpio was determined at 6 L/kg (2.34 mg/L aqueous exposure) and 3 L/kg (22.66 mg/L aqueous exposure) based on measured concentrations.

Executive summary:

The 8 wk-BCF in Cyprinus carpio was determined at 6 L/kg (2.34 mg/L aqueous exposure) and 3 L/kg (22.66 mg/L aqueous exposure) based on measured concentrations. In neither case there was any evidence that the accumulation increased with time. No mortality or other biological or behavioural abnormalities were found. No significant risk of bioaccumulation in fish was detected.

Description of key information

The 8 wk-BCF in Cyprinus carpio were determined at 6 L/kg (2.34 mg/L aqueous exposure) and 3 L/kg (22.66 mg/L aqueous exposure) based on measured concentrations. In neither case there was any evidence that the accumulation increased with time. No mortality or other biological or behavioural abnormalities were found. No significant risk of bioaccumulation in fish was detected.

Key value for chemical safety assessment

BCF (aquatic species):

6 L/kg ww