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EC number: 810-394-3
CAS number: 76326-99-3
Water Quality Parameters of the Dilution Water at the Start of the
Exposure (0 hours)
In the acute immobilisation test with Daphnia magna (STRAUS), the
effects of the limit concentration 133 mg/L of the test item Genamin DMG
75 , which is equivalent to 100 mg/L pure compound (100% (w/w) minus
water content of 24.8% (w/w)), were determined according to OECD 202
(2004) from 2016-04-19 to 2016-04-21 at the test facility.
The study was conducted under static
conditions over a period of 48 hours. Additionally, the limit
concentration was tested pH-adjusted.The pH adjustment reflects the
actually existing environmental conditions in the buffered aquatic
The test item Genamin DMG 75is a slightly yellowish solution,
which is soluble in water.
The limit concentration of 133 mg/L of the test item was mixed
thoroughly by manual agitation. Additionally, the pH of the limit
concentration wasadjusted from pH 9.27 to a pH of 7.00 by addition of 1
M HCl and tested under the same conditions as the not adjusted limit
concentration.Thelimit concentration (pH-adjusted and un-adjusted)was
visually clear throughout the exposure period.
Twenty daphnids (divided into 4 replicates with 5 daphnids each)
were exposed to the limit concentration (pH-adjusted and un-adjusted)
and the control.
The concentration of the test item Genamin DMG 75were analytically
verified via LC-MS/MS at the start of the exposure (0 hours) and at the
end of the exposure (48 hours) in the limit concentration (pH-adjusted
and un-adjusted) and in the control. Details of the analytical method
are presented in section11.
The measured concentrations of the test item were in the range of
91 to 95% of the nominal values at the start (0 hours) and at the end of
the exposure (48 hours).The analytical
results arepresented inTable 6.
The measured test item concentrations were all within ±20% of the
nominal concentration. This indicates that the test item concentration
was successfully maintained for the duration of the test. The effect
levels based on the nominal concentration of test item Genamin DMG 75
are given .
The validity criteria of the test guideline
In the limit
equivalent to 100 mg/L pure compound (100% (w/w) minus water content
of 24.8% (w/w)),no
effects onDaphnia magnawere observed.
EC10-, EC50-and EC100-Values
on the nominal concentration of the test itemGenaminDMG
Toxicity endpoint based on the
nominal concentration the test item [mg/L]
EC10 / 50 / 100
(100% (w/w) minus water content of 24.8% (w/w)
In the limit concentration 133 mg/L of the test item Genamin DMG 75,
which is equivalent to 100 mg/L pure compound (100% (w/w) minus water
content of 24.8% (w/w)), no effects on Daphnia magna were observed.
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