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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Remarks:
The guidelines of chemical testing good laboratory practices (HJ/T 155)”,
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
other: Gobiocypris rarus
Details on test organisms:
The Rare minnow, Gobiocypris rarus was used in the test on the basis of such important practical criteria as, for example, their ready availability throughout the year, ease of maintenance, convenience for testing and any relevant economic, biological or ecological factors. The test species of Rare minnow, Gobiocypris rarus (Batch No.: F20160215G), were obtained from a commercial fish supplier of Institute of Hydrobiology, Chinese Academy of Sciences.
Feeding fry were held at least for 12 days in holding tanks supplied with a continuous flow of aerated water before being used for testing. Fish to be used in the test were held for 7 days in water of the quality and temperature to be used in the test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
174 - 181 mg/L CACO3
Test temperature:
22.8 - 23.3 °C
pH:
7.62 - 7.76
Dissolved oxygen:
76 - 95 %
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
The results showed that under valid static test conditions, the 96 h-LC50 of Genamin DMG 75 to fish (Rare minnow, Gobiocypris rarus) is greater than the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L), while the maximum concentration causing no mortality (96 h-LC0) was equal to the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L). i.e.:
96 h-LC50 > 100 mg /L (measured water samples concentration was 102 mg/L);
96 h-LC0 = 100 mg/L (measured water samples concentration was 102 mg/L).
Executive summary:

Under static conditions,the acute toxicity of test substance(Genamin DMG 75)toRare minnow (Gobiocypris rarus)was conductedaccording to:“The guidelines for the testing of chemicals” (HJ/T 153-2004) and“The Guidelines for the Testing of Chemicals-Effects on Biotic Systems” (the 2nd edition.:Environment Press. 2013);with reference toProcedure 203 of theGuidelines for Testing of Chemicalsof the OECD:Fish, Acute Toxicity Test” (1992).

A range-finding test and then a limit test were performed respectively. Nominal concentrations of 1.00, 10.0 and 100mg/Lwere used in the range-finding test, while the nominal concentration of100 mg/Lwas used in the limit Test. Water samples taken from the control and the treatments in the limit test (0h, 24h, 48h, 72h & 96h) were analysed.Themean measured concentration of samples was 102mg/L.The analytical results showed that the concentration of the test substance was consistent in the test medium throughout the 96-hour test period(deviation within 20%). Thus a static procedure was reasonable.

In the range-finding test, 5 fish per treatment and no replicates were used. And 7 fishper treatmentwith 3 replicates were used in the limit Test.The testfishwereexposed for 96 hours to the testsolution.

During thelimittestperiod,the pHvaluesof the control mediumsand test mediums werebetween 7.62 and 7.76, the Dissolved Oxygen (DO) values varied from 76 % and 97 % of theair saturationat the test temperature, and the total hardness was in the range of 174 mg (CaCO3)/L to 181 mg (CaCO3)/L. During the test, the temperature of the test mediums were maintained in the range of 22.8 ºC to 23.2 ºC, and all fishes in the control group were normal. With thesameconditions, potassium dichromate (K2Cr2O7)wasused as thepositive controlsubstance, and the resulting 24 h-LC50was327mg/L.So the study met the acceptability criteria prescribed by the protocol and Test Guidelines (The mortality of control ≤ 10%;pH:6.0~8.5; dissolved oxygenconcentration:>60% of the air saturation value;total hardness:10~250 mg(CaCO3)/L; temperature: (23±2) ºC;24 h-LC50ofK2Cr2O7inthe range of 200 to 400 mg/L). Therefore the test was considered valid.

During the test period, all fishin the control groups and treated groupswere alive and appeared normal.The results showed that undervalidstatic test conditions, the 96 h-LC50ofGenamin DMG 75to fish (Rare minnow,Gobiocypris rarus)was greater than the nominal concentration of 100mg/L(measured water samples concentration was 102 mg/L), while the maximum concentration causing no mortality (96 h-LC0) was equal to the nominal concentration of 100mg/L(measured water samples concentration was 102 mg/L). i.e.:

96 h-LC50>100 mg/L(measured water samples concentration was 102 mg/L);

96 h-LC0=100 mg/L(measured water samples concentration was 102 mg/L).

Description of key information

The results showed that under valid static test conditions, the 96 h-LC50 of Genamin DMG 75 to fish (Rare minnow, Gobiocypris rarus) is greater than the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L), while the maximum concentration causing no mortality (96 h-LC0) was equal to the nominal concentration of 100 mg/L (measured water samples concentration was 102 mg/L). i.e.:

96 h-LC50 > 100 mg /L (measured water samples concentration was 102 mg/L);

96 h-LC0 = 100 mg/L (measured water samples concentration was 102 mg/L).

Key value for chemical safety assessment

Additional information