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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed to GLP and guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
10101-41-4
EC Number:
600-148-1
Cas Number:
10101-41-4
IUPAC Name:
10101-41-4
Constituent 2
Reference substance name:
calcium sulfate dihydrate
IUPAC Name:
calcium sulfate dihydrate
Details on test material:
- Name of test material (as cited in study report): Calcium sulfate dihydrate
- Analytical purity: 99.9%
- Lot/batch No.: Sigma Aldrich Corporation Lot. No. 109H0166

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: GSF Institute of Ecological Chemistry, Germany
- Age at study initiation: Juveniles (24 h old)



Study design

Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
124 mg/L as Ca CO3
Test temperature:
20.1 ± 0.2°C
pH:
7.90 - 7.98
Dissolved oxygen:
94 - 95%
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3


OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16/8 (light/dark)
- Light intensity: 1221 - 1996 lux


Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 79 mg/L
Nominal / measured:
nominal
Basis for effect:
other: No mortalities observed
Remarks on result:
other: The LC50 for calcium sulfate dihydrate was > 100 mg/L. Results have been corrected for calcium sulfate anhydrous
Details on results:
No immobilisation or mortality were observed

Applicant's summary and conclusion

Conclusions:
The LC50 of calcium sulfate anhydrous to Daphnia magna is >79 mg/L.