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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed to GLP and guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Calcium sulfate dihydrate
- Analytical purity: 99.9%
- Lot/batch No.: Sigma Aldrich Corporation Lot. No. 109H0166

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: GSF Institute of Ecological Chemistry, Germany
- Age at study initiation: Juveniles (24 h old)



Study design

Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
124 mg/L as Ca CO3
Test temperature:
20.1 ± 0.2°C
pH:
7.90 - 7.98
Dissolved oxygen:
94 - 95%
Nominal and measured concentrations:
100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3


OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16/8 (light/dark)
- Light intensity: 1221 - 1996 lux


Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 79 mg/L
Nominal / measured:
nominal
Basis for effect:
other: No mortalities observed
Remarks on result:
other: The LC50 for calcium sulfate dihydrate was > 100 mg/L. Results have been corrected for calcium sulfate anhydrous
Details on results:
No immobilisation or mortality were observed

Applicant's summary and conclusion

Conclusions:
The LC50 of calcium sulfate anhydrous to Daphnia magna is >79 mg/L.