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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed to GLP and guideline

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Calcium sulfate dihydrate
- Analytical purity: 99.9%
- Lot/batch No.: Sigma Aldrich Lot. No. -109H0166

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Japanese rice fish
- Age at study initiation: 5 months
- Length at study initiation: 2.7 ± 0.1 cm
- Weight at study initiation: 0.15 ± 0.02 g
- Feeding during test: No food was fed before 1 day prior to testing and during the test



ACCLIMATION
- Acclimation period: 7 days


Study design

Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
54.5 mg/L as CaCO3
Test temperature:
242 - 24.8°C
pH:
7.41 - 7.95
Dissolved oxygen:
81.9 - 105.3%
Nominal and measured concentrations:
Limit test performed at a concentration 100 mg/L
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 7 (no replicates)
- Biomass loading rate: 8.7 L aquarium per 7 fish



OTHER TEST CONDITIONS
- Photoperiod:16/8 (light/dark)
- Light intensity: 1628 - 1816 lux


Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 79 mg/L
Nominal / measured:
nominal
Basis for effect:
other: No mortalities observed
Remarks on result:
other: The LC50 for calcium sulfate dihydrate was >100 mg/L the result has been corrected for calcium sulfate anhydrous
Details on results:
There were no mortalities and no symptoms of intoxication were observed throughout the study.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of calcium sulfate anhydrous is > 79 mg/L