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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August 1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no
Remarks:
not necessary

Test material

Constituent 1
Chemical structure
Reference substance name:
2,6-dihydroxy-4-methyl-1-[3-(methylazaniumyl)propyl]pyridin-1-ium sulfate
EC Number:
931-673-7
Cas Number:
1333469-72-9
Molecular formula:
C10 H18 N2 O6 S1
IUPAC Name:
2,6-dihydroxy-4-methyl-1-[3-(methylazaniumyl)propyl]pyridin-1-ium sulfate
Test material form:
liquid

Test solutions

Vehicle:
no
Remarks:
Direct dispersion in water
Details on test solutions:
The sample was maintained at 21ºC with continous aeration and used on the day of collection. The pH was buffered to pH 7.2 with sodium hydroxyde solution and the suspended solids level equal to 3.6 g/L prior to use.

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
Mixed population of activated sewage sludge micro-organisms.
Source: the aeration Stage of Severn Trent PLC Sewage Treatment Plant, Belper, Derbyshire (UK).

Study design

Test type:
not specified
Water media type:
not specified
Total exposure duration:
3 h

Test conditions

Test temperature:
21ºC
Nominal and measured concentrations:
range finding study, nominal: 1.0, 10, 100, 1000 mg/L (1 replicate per concentration)
definitive study, nominal: 100 mg/L (3 replicates)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: % inhibition O2 consumption
Results with reference substance (positive control):
EC50 (3 hour contact time) = 12.5 mg/L

Any other information on results incl. tables

Sludge - range finding study


 























































 



Concentration (mg/L)



O2 consumption rates, mg O2/L/min


3-hour contact time



% Inhibition



Control



R1



0.35



-



R2



0.34



-



Test



1.0



0.37



<7>



10



0.36



<4>



100



0.37



<7>



1000



0.00



100



Reference



3.2



0.26



25



32



0.06



83



 


 


<increase>


Variation between controls: +-1%


 


 


 


 


 


Sludge - definitive study


 


















































 



Concentration (mg/L)



O2 consumption rates, mg O2/L/min


3-hour contact time



% Inhibition



Control



R1



0.37



-



R2



0.36



-



Test



100 R1



0.38



<4>



100 R2



0.36



1



100 R3



0.36



1



Reference



3.2



0.37



<1>



32



0.05



86



 


<increase>


Variation between controls: +-1%


R1-R3: replicates R1-R3


 

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
HYMAP: EC50 (3h contact time) > 100 mg/L