Pyridinium, 2,6-dihydroxy-4-methyl-1-[3-(methylamino)propyl]-, sulfate (1:1)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August-September 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Remarks:

not necessary

Test material

Test material information

Constituent 1

Reference substance name:

2,6-dihydroxy-4-methyl-1-[3-(methylazaniumyl)propyl]pyridin-1-ium sulfate

EC Number:

931-673-7

Cas Number:

1333469-72-9

Molecular formula:

C10 H18 N2 O6 S1

IUPAC Name:

2,6-dihydroxy-4-methyl-1-[3-(methylazaniumyl)propyl]pyridin-1-ium sulfate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Remarks:

direct dispersion in water

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Strain: not specified
- Source: Parkwood Trout Farm, Kent, U.K.
- Age at study initiation (mean and range, SD): not specified
- Length at study initiation (length definition, mean, range and SD): 4.9+-0.2 cm
- Weight at study initiation (mean and range, SD): 1.36+-0.22 g
- Method of breeding: not specified
- Medication: none

ACCLIMATION
- Acclimation period: 7 days prior to the start of the study
- Health during acclimation (any mortality observed): no mortalities observed in the 7 days prior to the start of the study

Study design

Test type:

other: daily renewal of the media

Water media type:

other: dechlorinated tap water (sodium thiosulphate)

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

approximately 50 mg/L as CaCO3

Test temperature:

14ºC

Nominal and measured concentrations:

Range finding study (3 fish): 1.0, 10, 100 mg/L
Definitive study (10 fish): two replicates 100 mg/L

Details on test conditions:

test vessels: 20 L
aeration: yes

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 1. Results Range finding study (24-28 August 1992)

Concentration (mg/L)	Cumulative mortality (initial population=3)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
1.0	0	0	0	0
10	0	0	0	0
100	0	0	0	0

 

Table 2. Results of the definitive study (1-5 September 1992)

Concentration (mg/L)	Cumulative mortality (initial population=10)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
100 R1	0	0	0	0
100 R2	0	0	0	0

R1-R2: replicates R1, R2

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The test item HYMAP has a LC50 (96h) for fish > 100 mg/L