Ethyl enantate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26.05.1999 - 28.05.1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Analysis of the test concentration and a control at test start and after 48 hours of exposure.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS, parthenogenetic females
- Strain: cloned at the Federal Health Office (BGA) in Berlin
- Source: laboratory bred at Bayer AG Lever- kusen
- Age at study initiation: 0 - 24 hours
- Keeping water: M4-Medium according to Elendt and BGA (1992)

Separation of test animals according to size before start of test:
- 1st sieving on : 25.05.1999 at 2.00 p.m.
- 2nd sieving on : 26.05.1999 at 8.00 a.m.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

14.6 °dH

Test temperature:

20.15 °C
Remark: The values of the abiotic parameters (oxygen, pH and temperature) were determined from the total volume of the parallel solutions.

pH:

7.7
Remark: The values of the abiotic parameters (oxygen, pH and temperature) were determined from the total volume of the parallel solutions.

Dissolved oxygen:

48h:
- control: 8.1 mg/L
- test substance: 3.7 mg/L
Remark: The values of the abiotic parameters (oxygen, pH and temperature) were determined from the total volume of the parallel solutions.

Nominal and measured concentrations:

24 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: "Karlsruher bottles" with stoppers
- Material, size, headspace, fill volume: 250 - 300 mL test medium, not ventilated, had space
- N° parallel solutions: 2
- N° dahnia per vessel: 10

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark

TEST CONCENTRATIONS
- Test concentration: 24 mg/L
- Solubilizer or other: ultra-turrax magnetic stirrer folded filter

CONTROL
- M4-Medium according to Elendt and BGA (1992)

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 24 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 26.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Analysis of test concentration

All values refer to TOC determination. According to the relevant product informations, 1 mg/L TOC equals to 1.5 mg/L of the test substance.

Nominal concentration test substance (mg/L)	Results by start of test and after 48 hours (mg/L)  0 hours  48 hours
Control	< 2  < 2
24	15  20

Validity criteria fulfilled:

yes

Conclusions:

The EC0 for 48 hours was found to be >=26.3 mg/L.

Executive summary:

The short-term toxicity of the test item to Daphnia was assessed in an acute immobilisation test according to OECD 202. The study is performed according to GLP.

A defined concentration of the test substance (24 mg/L) was used and the study lasted 48 hours. The immobilisation of the Daphnia was assessed at the end of the study period. The EC 0 was determined directly from the study. No effect on the immobilisation was found. The EC0 for 48 hours was found to be >=26.3 mg/L.

Description of key information

No effect on the immobilisation was found. The EC0 for 48 hours was found to be ≥ 26.3 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

26.3 mg/L

Additional information

There is one study available assessing the short-term toxicity of the test item to Daphnia in an acute immobilisation test according to OECD 202. The study is performed according to GLP.

The test substance (24 mg/L) is assessed for 48 hours. The immobilisation of the Daphnia was assessed at the end of the study period. The EC 0 was determined.

No effect on the immobilisation was found. The EC0 for 48 hours was found to be >=26.3 mg/L.