2-ethylhexyl acrylate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study report

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1950

Report date:

1950

Reference 2

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List IV.

Author:

Smyth HF et al.

Year:

1951

Bibliographic source:

AMA Arch. Ind. Hyg. Occup. Med. 4: 119-122

Materials and methods

Principles of method if other than guideline:

Range-finding toxicity test basically according to the method described by Smyth HF Jr. and Carpenter CP (1948).

Smyth HF Jr. and Carpenter CP (1948). Further Experience with the Range-Finding Test in the Industrial Toxicology Laboratory. J. Ind. Hyg. Toxicol. 30: 63-68

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhexyl acrylate

EC Number:

203-080-7

EC Name:

2-ethylhexyl acrylate

Cas Number:

103-11-7

Molecular formula:

C11H20O2

IUPAC Name:

2-ethylhexyl acrylate

Details on test material:

- Test substance: 2-ethylhexyl acrylate
- Chemical name: 2-propenoic acid, 2-ethylhexyl ester
- Analytical Purity: no data
- Density: 0.887 g/cm3 (DIN 51757, cited in EU RAR, 2005)

Test animals

Species:

rabbit

Strain:

not specified

Sex:

not specified

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Type of wrap: impervious "Vinylite" sheeting


REMOVAL OF TEST SUBSTANCE
- Washing: yes
- Time after start of exposure: 24 h


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5.0 and 10.0 mL/kg bw, respectively

Duration of exposure:

24 h

Doses:

5.0 and 10.0 mL/kg bw (corresponding to 4435 and 8870 mg/kg bw)

No. of animals per sex per dose:

4

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days

- Test conditions and method according to Smyth HF Jr. and Carpenter CP (1948):
Penetration of rabbit skin was estimated by a technique closely akin to the one-day cuff method of Draize et al. (1944) using groups of at least 6 rabbits. The fur was removed from the entire trunk by clipping, and the dose was retained beneath an impervious plastic film. Dosages greater than 20 mL/kg bw could not be retained in contact with the skin. The animals were immobilized during the 24-hour contact period, after which the film was removed and the rabbits were caged for the subsequent 14-day observation period. In this study, 8 rabbits were dosed by introduction of the undiluted test substance under impervious "Vinylite" sheeting which covered their clipped trunks. The LD50 value was determined by Thompson's method.

Statistics:

The LD50 value was determined by Thompson's method.

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

7 522 mg/kg bw

Mortality:

Original value: LD50 = 8.480 mL/kg
Based on a densitiy of 0.887 g/mL, LD50 = 7522 mg/kg bw.

3/4 animals succumbed to a dosage of 10 mL/kg bw (= 8870 mg/kg bw), and 4/4 survived 5.0 mL/kg bw (= 4435 mg/kg bw). The estimated LD50 calculated by Thompson's method after assuming 100 % mortality at 20 mL/kg and 100 % survival at 2.52 mL/kg bw was 8.48 mL/kg bw.

Clinical signs:

other: no data

Gross pathology:

no data

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Executive summary:

Two groups of 4 rabbits were dosed by introduction of the undiluted ethylhexyl acrylate under impervious "Vinylite" sheeting which covered their clipped trunks. Three of 4 succumbed to a dosage of 10. ml./kg and 4 survived 5.0 ml./kg.

The estimated R.F. LD50 for rabbits by skin penetration is 8.48 ml/kg (7522 mg/kg) for the undiluted material.