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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
before 1984
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: No adequate test system.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1984

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Primary root tips of the structurally reconstructed karyotype ACB of Vicia faba were used. They were treated with succinic acid under the following conditions: 1 h treatment, 4 x 10-3 M. Additionally, a 2 h treatment with 1 x 10-3 M hydrazine was performed. Chromosome aberration frequencies were determined.
GLP compliance:
not specified
Type of assay:
other: clastogenic effects on primary root tips of Vicia faba were investigated

Test material

Constituent 1
Chemical structure
Reference substance name:
Succinic acid
EC Number:
203-740-4
EC Name:
Succinic acid
Cas Number:
110-15-6
Molecular formula:
C4H6O4
IUPAC Name:
butanedioic acid

Method

Details on test system and experimental conditions:
Primary root tips (approximately 3 cm in length) of the structurally reconstructed karyotype ACB of Vicia faba (cf. Michaelis and Rieger, 1971) were used. They were treated with succinic acid under the following conditions: 1 h treatment, 4 x 10-3 M. Additionally, a 2 h treatment with 1 x 10-3 M hydrazine was performed. After treatment and recovery in running tap water (recovery times: 21 h, 24 h) the roots were exposed to 0.05% colchicine for 2 h, fixed in ethanol/glacial acetic acid (3:1), and hydrolyzed (11 min at 60°C in HCI). Permanent Feulgen squashes were made by the dry-ice method. Aberration frequencies were determined by scoring the following types of chromatid aberrations in metaphases of first post treatment mitoses: isochromatid breaks, duplication deletions, intercalary deletions, and reciprocal chromatid translocations. Gaps were omitted. The localization of chromatid aberrations was based on a subdivision of the haploid chromosome complement into 28 segments.

Results and discussion

Additional information on results:
Succinic acid proved incapable of inducing aberrations. The aberration yield was 0.02 aberrations/cell, a frequency corresponding to the spontaneous level.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

Succinic acid proved under the given conditions incapable of inducing aberrations. The aberration yield was 0.02 aberrations/cell, a frequency corresponding to the spontaneous level.
Executive summary:

Primary root tips (approximately 3 cm in length) of the structurally reconstructed karyotype ACB of Vicia faba (cf. Michaelis and Rieger, 1971) were used. They were treated with succinic acid under the following conditions: 1 h treatment, 4 x 10 -3 M. Additionally, a 2 h treatment with 1 x 10 - 3 M hydrazine was performed. After treatment and recovery in running tap water (recovery times: 21 h, 24 h) the roots were exposed to 0.05% colchicine for 2 h, fixed in ethanol/glacial acetic acid (3:1), and hydrolysed (11 min at 60°C in HCI). Permanent Feulgen squashes were made by the dry-ice method. Aberration frequencies were determined by scoring the following types of chromatid aberrations in metaphases of first post treatment mitoses: isochromatid breaks, duplication deletions, intercalary deletions, and reciprocal chromatid translocations. Gaps were omitted. The localization of chromatid aberrations was based on a subdivision of the haploid chromosome complement into 28 segments.

Succinic acid proved under the given conditions incapable of inducing aberrations. The aberration yield was 0.02 aberrations/cell, a frequency corresponding to the spontaneous level.