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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 1-3, 1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EPA-660/3-75-009, "Methods for acute toxicity with fish, macrovertebrates, and amphibians"
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate
EC Number:
225-948-4
EC Name:
Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate
Cas Number:
5165-97-9
Molecular formula:
C7H13NO4S.Na
IUPAC Name:
sodium 2-(acryloylamino)-2-methylpropane-1-sulfonate
Details on test material:
- Name of test material (as cited in study report): OS #48933F
- Physical state: liquid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: 100%
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: stable
- Storage condition of test material: no data

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution
- Chemical name of vehicle: water

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnid
- Strain: Daphnia magna
- Source: in house
- Age at study initiation: ≤24 hours
- Weight at study initiation: no data
- Feeding during test: no
ACCLIMATION
- Acclimation period: yes
- Acclimation conditions: same as test
- Type and amount of food: green algae (Ankistrodemus sp.) and yeast
- Feeding frequency: once daily
- Health during acclimation (any mortality observed): no data

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
160 - 180 mg/L as CaCO3
Test temperature:
20±1°C
pH:
7.9 - 8.3
Dissolved oxygen:
>60% saturation
Nominal and measured concentrations:
Nominal concentrations: control; 130, 220, 360, 600 and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: beaker containing each 200 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water fortified according to test protocol and filtered through a carbon filter and an Amberlite ZAD-7 resin column to remove any potential organic contaminants
- Alkalinity: 110- 130 mg/L
- Conductivity: 400 - 600 microhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8-hours of darkness
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
There was no effect on Daphnia magna at the highest test concentration of 1000 mg/l.

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