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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Stability and solubility of the test and reference substances were not studied.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
2-Acrylamido-2-methylpropanesulfonic acid, ammonium salt
IUPAC Name:
2-Acrylamido-2-methylpropanesulfonic acid, ammonium salt
Constituent 2
Reference substance name:
Ammonium 2-methyl-2-[(1-oxo-2-propenyl)amino]-1-propanesulfonate
EC Number:
421-680-9
EC Name:
Ammonium 2-methyl-2-[(1-oxo-2-propenyl)amino]-1-propanesulfonate
IUPAC Name:
421-680-9
Constituent 3
Reference substance name:
58374-69-9
EC Number:
611-646-3
Cas Number:
58374-69-9
IUPAC Name:
58374-69-9
Details on test material:
- Name of test material (as cited in study report): OS 114454
- Substance type: organic
- Physical state: liquid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test concentrations for the test substance were 1, 10, 100, 1,000 and 10,000 ppm (mg/l). The test substance was water-soluble. No organic solvents were used to facilitate the dispersion of the test substance. The test mixtures were well agitated by air flow at all times, and for test flasks with 1,000 and 10,000 ppm concentrations, magnetic stirring was employed to ensure dispersion of the test substance.

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
The inoculum used was the return sludge collected from a local waste-water treatment plant (Mentor, Ohio). On return to the laboratory the sludge was centrifuged and the supernatant decanted. The sludge was then washed in water three times by re-suspension and centrifugation. A small amount of the washed sludge was dried and weighed. From this result the amount of wet sludge was calculated to be suspended in water in order to obtain an activated sludge with a mixed liquor suspended solids level of 4 g/l (±10%). This level gave a concentration of 1.6 g/l in the test medium. The prepared sludge was maintained by continuous aeration and by addition of 30-50 ml synthetic sewage feed per litre of suspended sludge at the end of every working day.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
10 minutes

Test conditions

Hardness:
No specified
Test temperature:
Ambient (not monitored)
pH:
Not specified
Dissolved oxygen:
Not specified
Salinity:
Not specified
Nominal and measured concentrations:
Nominal concentrations: 1, 10, 100, 1,000 and 10,000 ppm (mg/l) of test substance
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): closed
- Size, fill volume: 1 litre flasks filled to 500 ml
- Aeration: continuous oil-free air, 0.5-1.0 litre/minute
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 200 ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Distilled water

OTHER TEST CONDITIONS
- Adjustment of pH: no

EFFECT PARAMETERS MEASURED: respiration rate (mg O2/l.hr)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: x10
- Range finding study: no
Reference substance (positive control):
yes
Remarks:
3,5 dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: EC50/3 hours was in the accepted range of 5 to 30 mg/l

Any other information on results incl. tables

Activated Sludge, Respiration Inhibition Effect of OS 114454

   Concentration(ppm) Log Concentration (ppm) Respiration Rate (mg 02/l.hr) Inhibition(%)
Control 1 -  - 42.1 -  
Control 2 - - 40.7 -   
Test A-1 4 0.60 40.0 3
Test A-10 12 1.08 40.7 2
Test A-100 110 2.04 40.0 3
 Test A-1000 1,022 3.01 44.4 -7
 Test A-10000 10,000 4.00 41.4 0
 Ref 10 10 1.00 25.5 38
 Ref 20 20 1.30 9.8 76
 Ref 40 40 1.60 5.5 87

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance, up to the concentration of 10,000 ppm, did not show significant toxicity toward the microbial population. The EC50 was estimated to be >10,000 ppm and the NOEC estimated to be at 10,000 ppm.