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EC number: 225-948-4 | CAS number: 5165-97-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Stability and solubility of the test and reference substances were not studied.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-Acrylamido-2-methylpropanesulfonic acid, ammonium salt
- IUPAC Name:
- 2-Acrylamido-2-methylpropanesulfonic acid, ammonium salt
- Reference substance name:
- Ammonium 2-methyl-2-[(1-oxo-2-propenyl)amino]-1-propanesulfonate
- EC Number:
- 421-680-9
- EC Name:
- Ammonium 2-methyl-2-[(1-oxo-2-propenyl)amino]-1-propanesulfonate
- IUPAC Name:
- 421-680-9
- Reference substance name:
- 58374-69-9
- EC Number:
- 611-646-3
- Cas Number:
- 58374-69-9
- IUPAC Name:
- 58374-69-9
- Details on test material:
- - Name of test material (as cited in study report): OS 114454
- Substance type: organic
- Physical state: liquid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified
Constituent 1
Constituent 2
Constituent 3
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test concentrations for the test substance were 1, 10, 100, 1,000 and 10,000 ppm (mg/l). The test substance was water-soluble. No organic solvents were used to facilitate the dispersion of the test substance. The test mixtures were well agitated by air flow at all times, and for test flasks with 1,000 and 10,000 ppm concentrations, magnetic stirring was employed to ensure dispersion of the test substance.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The inoculum used was the return sludge collected from a local waste-water treatment plant (Mentor, Ohio). On return to the laboratory the sludge was centrifuged and the supernatant decanted. The sludge was then washed in water three times by re-suspension and centrifugation. A small amount of the washed sludge was dried and weighed. From this result the amount of wet sludge was calculated to be suspended in water in order to obtain an activated sludge with a mixed liquor suspended solids level of 4 g/l (±10%). This level gave a concentration of 1.6 g/l in the test medium. The prepared sludge was maintained by continuous aeration and by addition of 30-50 ml synthetic sewage feed per litre of suspended sludge at the end of every working day.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- 10 minutes
Test conditions
- Hardness:
- No specified
- Test temperature:
- Ambient (not monitored)
- pH:
- Not specified
- Dissolved oxygen:
- Not specified
- Salinity:
- Not specified
- Nominal and measured concentrations:
- Nominal concentrations: 1, 10, 100, 1,000 and 10,000 ppm (mg/l) of test substance
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): closed
- Size, fill volume: 1 litre flasks filled to 500 ml
- Aeration: continuous oil-free air, 0.5-1.0 litre/minute
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 200 ml
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Distilled water
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: respiration rate (mg O2/l.hr)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x10
- Range finding study: no - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC0
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50/3 hours was in the accepted range of 5 to 30 mg/l
Any other information on results incl. tables
Activated Sludge, Respiration Inhibition Effect of OS 114454
Concentration(ppm) | Log Concentration (ppm) | Respiration Rate (mg 02/l.hr) | Inhibition(%) | |||||
Control 1 | - | - | 42.1 | - | ||||
Control 2 | - | - | 40.7 | - | ||||
Test A-1 | 4 | 0.60 | 40.0 | 3 | ||||
Test A-10 | 12 | 1.08 | 40.7 | 2 | ||||
Test A-100 | 110 | 2.04 | 40.0 | 3 | ||||
Test A-1000 | 1,022 | 3.01 | 44.4 | -7 | ||||
Test A-10000 | 10,000 | 4.00 | 41.4 | 0 | ||||
Ref 10 | 10 | 1.00 | 25.5 | 38 | ||||
Ref 20 | 20 | 1.30 | 9.8 | 76 | ||||
Ref 40 | 40 | 1.60 | 5.5 | 87 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance, up to the concentration of 10,000 ppm, did not show significant toxicity toward the microbial population. The EC50 was estimated to be >10,000 ppm and the NOEC estimated to be at 10,000 ppm.
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