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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
September 1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted according to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EPA-660/3-75-009, "Methods for acute toxicity tests with fish, macrovertebrates, and amphibians.
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate
EC Number:
225-948-4
EC Name:
Sodium 2-methyl-2-[(1-oxoallyl)amino]propanesulphonate
Cas Number:
5165-97-9
Molecular formula:
C7H13NO4S.Na
IUPAC Name:
sodium 2-(acryloylamino)-2-methylpropane-1-sulfonate
Details on test material:
- Name of test material: 2-Acrylamido-2-methylpropanesulfonic acid, sodium slat
- Analytical purity: 50%
- Physical state: liquid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution
- Chemical name of vehicle: soft water reconstituted from deionized water
- Stock solution: 150 mg/mL
- Final test solutions: 130, 220, 360, 600 and 1000 mg/L

Test organisms

Test organisms (species):
Lepomis macrochirus
Details on test organisms:
TEST ORGANISM
- Common name: Bluegill
- Source: in house laboratory supply (Lot # 83A13)
- Age at study initiation: juvenile
- Length at study initiation: 3.8 cm
- Weight at study initiation: 0.66 g (wet weight)
- Feeding during test: no
ACCLIMATION
- Acclimation period: 14 days prior to start the test
- Acclimation conditions: same as test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
44 mg/L as CaCO3
Test temperature:
22°C
pH:
7.5
Dissolved oxygen:
3.1 to 8.7 mg 02/L (35 to 99% of saturation)
Nominal and measured concentrations:
Nominal concentration:
Control, 130; 220; 360; 600 and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: glass exposure vessels containing 15 L of test solution
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): no replicates

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: soft water reconstituted from dionized water
- Alkalinity: 32 mg/L
- Conductivity: 140 microhos/cm

OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours darkness

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6 - 1.7
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Abnormal responses: None

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC0 at 96 hours for fish of the sodium salt of ATBS was the highest test concentration of 1000 mg/l.