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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
5 December 2000 to 7 December 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0 and 100 mg/L
- Sampling method: samples "drawn up" from containers
- Sample storage conditions before analysis: 0 hour samples taken immediately after preparation
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: weighed amount (20 mg) of dimethylcarbonate were added to 200 mL of reconstituted water in order to obtain the final nominal concentration of 100 mg/L
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution):
- Evidence of undissolved material (e.g. precipitate, surface film, etc):
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: not stated
- Strain: not stated
- Source: bred in the laboratory
- Age at study initiation (mean and range, SD): not more than 24 h
- Weight at study initiation (mean and range, SD): not stated
- Length at study initiation (length definition, mean, range and SD): not stated
- Valve height at study initiation, for shell deposition study (mean and range, SD): not stated
- Peripheral shell growth removed prior to test initiation: not stated
- Method of breeding: not stated
- Feeding during test
- Food type: green algae (Selenastrum capricornutum)
- Amount: not stated
- Frequency: daily (animals not fed during treatment period)


ACCLIMATION
- Acclimation period: none - animals cultured in water similar to treatment water
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):


QUARANTINE (wild caught)
- Duration:
- Health/mortality:
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
Less than 250 mg/L calcium carbonate
Test temperature:
20/21C
pH:
7.6/7.9
Dissolved oxygen:
8.6/9.0 mg/L dissolved oxygen
Salinity:
not measured
Nominal and measured concentrations:
Nominal concentations: 0 and 100 mg/L

Time h Nominal concentration DMC mg/L Measured concentration DMC mg/L
0 100 82.29
48 without Daphniae 100 84.33
48 with Daphniae 100 66.03
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): small (10/15 mL) glass containers
- Type of flow-through (e.g. peristaltic or proportional diluter): static test
- Renewal rate of test solution (frequency/flow rate): static test
- No. of organisms per vessel: 20 animals/concentration subdivided into batches of five animals each in small closed glass containers


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water used was that suggested by ISO 6341-1982
- Total organic carbon: not stated
- Particulate matter: not stated
- Metals: not stated
- Pesticides: not stated
- Chlorine: not stated
- Alkalinity: not stated
- Ca/Mg ratio: 4:1
- Conductivity: Not stated
- Culture medium different from test medium: stated to be similar
- Intervals of water quality measurement: 0 and 48 h


OTHER TEST CONDITIONS
- Adjustment of pH: not stated
- Photoperiod:8/16 h of dark/light daily
- Light intensity: not stated


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :


TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: immobilisation
Details on results:
None
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
EC50 and its statistical limits: by the trimmed Spearman-Karber Method (Hamilton,
M.A., R.C. Russo, and R.V. Thurston, 1977. Trimmed Spearman-Karber method for
estimating medium lethal concentrations in toxicity bioassays. Environ. Sci. Technol,
11(7): 714-719; correction 12(4): 27(1978).

Cumulative immobilisation

 

mg/L

24 h

48 h

 

No. immobilised animals

% immobilisation

No. immobilised animals

% immobilisation

0

0

0

1

5

100

2

10

5

25

Validity criteria fulfilled:
yes
Conclusions:
Experimental data from an acute immobilization test in which Daphnia magna were treated with the test article DIMETHYLCARBONATE at the nominal
concentration of 100 mg/L are given in this report. The animals were treated and observed for 48 hours .
One animal was immobilized in the control group at 48 h observation time.
Group 1: 2 and 5 animals were immobilized at 24 and 48 hours respectively.
The ECso of DIMETHYLCARBONATE at 24 and at 48 hours were >100 mg/L, (83.30 and 74.16 mg/L. measured concentrations).

Description of key information

Experimental data from an acute immobilization test in which Daphnia magna were treated with the test article Dimethyl carbonate at the nominal concentration of 100 mg/L are given in this report. The animals were treated and observed for 48 hours . One animal was immobilized in the control group at 48 h observation time.
The 48h EC50 has been determined to be >100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information