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Toxicity to microorganisms

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Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
Principles of method if other than guideline:
The respiration inhibition of the test substance to a sludge concentration of 1.5 g/L suspended solids is recorded after 3 hours.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge
Details on inoculum:
- Initial biomass concentration: 1.5 g/L suspended solids
- Source: Rungsted municipal treatment plant, Denmark, treating almost exclusively domestic sewage
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Reference substance (positive control):
not specified
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
135 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
188 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Principles of method if other than guideline:
6 bacteria preselected from domestic and industrial effluents, cultivation for 3 hours in BOD bottle with toxicant
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
other: synthetic activated sludge
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
480 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Endpoint:
toxicity to microorganisms
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
weight of evidence
Study period:
12 Nov - 15 Nov 1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture: Stamm Berlin 33/2 (DSM 50026)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Post exposure observation period:
not relevant
Test temperature:
21 °C
pH:
6.6 - 6.8
Nominal and measured concentrations:
nominal: 0, 1000, 3000 and 10000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Clim-o-shake at 100 rpm

EFFECT PARAMETERS MEASURED: growth inhibition, measured photometrically at 436 nm (Zeiss filterphotometer PL4)
Reference substance (positive control):
no
Duration:
16 h
Dose descriptor:
EC0
Effect conc.:
409 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition
Duration:
16 h
Dose descriptor:
EC10
Effect conc.:
1 083.85 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition

0.58% inhibition at 1000 mg prod./L

15.3% inhibition at 3000 mg prod./L

60.63% inhibition at 10000 mg prod./L

Endpoint:
activated sludge respiration inhibition testing
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
Reason / purpose for cross-reference:
read-across source
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
135 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other:
Remarks:
Source, key, 151-21-3, 1983

Description of key information

3h EC50 (activated sludge, domestic) = 135 mg/L (respiration inhibition test, similar to OECD 209)

Key value for chemical safety assessment

EC50 for microorganisms:
135 mg/L

Additional information

Since no reliable studies on toxic effects of C12-14AS NH4 (CAS 90583-11-2) to microorganisms are available, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5, a read-across to another structurally related category members was conducted.

The key study was performed with C12AS Na (CAS 151-21-3) with activated sludge from a municipal sewage treatment plant (Dutka 1983). The non-GLP test was performed according to a guideline similar to OECD 209. The EC50 values of 188 and 135 mg/L (nominal concentrations) were obtained for the endpoint respiration inhibition after 30 minutes and 3 hours of exposure, respectively. Since preference is given to tests with a mixed inoculum, rather than tests based on single species, the 3-hour EC50 value of 135 mg/L was used for the PNEC STP derivation for C12-14AS NH4 (CAS 90583-11-2).

In the supporting non-GLP study with CAS 151-21-3, Dutka and Kwan (1984) used synthetic activated sludge and measured respiration rate after 3 hours. The 3h IC50 value was 480 mg/L (nominal concentration). Moreover, a GLP test according to DIN 38412 part 8 with Pseudomonas putida using C12-14AS TEA (CAS 90583-18-9) is available (Guhl 1992). In this study, the growth inhibition of Pseudomonas putida was recorded after approximately 16 hours of exposure. The 16-h EC10 value was determined to be 1084 mg a.i./L (nominal concentration).