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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Refer to the Category Approach Justification document provided in IUCLID6 Section 13
Reason / purpose for cross-reference:
read-across source
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Source, key, CAS 85586-07-8, 1995
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
01 Mar - 03 Mar 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other: EG-guideline EG/92/69/EWG
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0, 0.6, 1.3, 2.5, 5.0 and 10.0 mg/L
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: waterflea
- Strain: Clone 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): <24 hours
- Method of breeding: Daphnids held in 1 L beakers with M4 Medium (Elendt), every 2-3 days juveniles are removed and water changed, every 4 weeks a generation change is induced by continuing the breeding with juveniles only
- Feeding during test: no

ACCLIMATION (in-house culture conditions)
- Type and amount of food: Scenedesmus subspicatus ad libidum
- Feeding frequency: daily



Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
no data
Test temperature:
20 ± 1 °C
pH:
Test end: 7.8 - 8.3
Dissolved oxygen:
Test end: 7.2 - 8.2 mg/L
Nominal and measured concentrations:
Nominal: 0, 0.6, 1.3, 2.5, 5.0 and 10.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 mL hourglasses
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility, daily
Reference substance (positive control):
no
Remarks:
Reference substances potassium dichromate was tested in a separate study
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
4.7 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: C.L.: 2.5 - 5.0 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Results with reference substance (positive control):
Potassium dichromate was tested as reference substance in a separate test (11 Jan 1995)
- Results with reference substance valid? yes
- Mortality: 100% immobilised at 2 mg/L; 35% immobilised at 1 mg/L
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
28 Feb - 02 Mar 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 1
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 2.6 g test substance weighed into 1 L VE water as stock solution
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Clone 5
- Source: in-house culture
- Age at study initiation: <24 hours
- Feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 ± 1 °C
Nominal and measured concentrations:
Nominal: 0.6, 1.3, 2.5, 5.0 and 10.0 mg/L
Geometrical mean (analytics): <20% variation from nominal values
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 mL hourglasses
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL/daphnid

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: VE water

OTHER TEST CONDITIONS
- Photoperiod: 24 hours darkness

EFFECT PARAMETERS MEASURED: immobilisation after 24 and 48 h

Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: 5.0-10.0 (C.L.)
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: 35% daphnids immobilised at 1 mg/L potassium dichromate; 100% daphnids immobilised at 2 mg/L potassium dichromate

Table 1: Cumulative number and % immobile daphnids

Concentration 48 h 48 h 48 h
mobile immobile immobile [%]
Control 20 0 0
0.6 19 1 5
1.3 20 0 0
2.5 20 0 0

Description of key information

48h EC50 (Daphnia magna) = 4.7 mg/L (nominal concentration, EG Guideline 92/69/EWG)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
4.7 mg/L

Additional information

Since no reliable short-term test involving aquatic invertebrates is available for C12-14AS NH4 (CAS 90583-11-2), in accordance to Regulation (EC) 1907/2006 Annex XI, 1.5, a read-across to other structurally related category members was conducted. Read-across data from the following substances were used: C12-14AS Na (CAS 85586-07-8) and C12-14AS TEA (CAS 90583-18-9). Toxicity of alkyl sulfates is dependent on the carbon chain length and the influence of the counter ion can be neglected.

The key, GLP study is available for C12-14AS Na (CAS 85586-07-8) according to EG Guideline 92/69/EWG (Scholz 1995a). In this study Daphnia magna was exposed in a static system to five concentrations up to 10 mg/L (nominal concentration corresponding to 9.5 mg/L of measured concentration) of C12-14AS Na of 29.8% purity (in water). The 48-hour EC50 value of 4.7 mg a.i./L (nominal concentration) could be determined. Composition of carbon chains of the tested category member C12-14AS Na (CAS 85586-07-8) is the same as of C12-14AS NH4 (CAS 90583-11-2): both contain on average 65% of C12 and 35% of C14 chains. Due to structural and property similarity with the tested category member, the 48-hour EC50 value of 4.7 mg/L is considered as a reliable for C12-14AS NH4 (CAS 90583-11-2).

Moreover, one reliable study investigating toxic effects of C12-14AS TEA (CAS 90583-18-9) to Daphnia magna is available. The GLP study was performed according to DIN 38412, part 1, equivalent to OECD 202 (Scholz 1995b). Inhibition of mobility of daphnids caused by the test substance was determined after 24 and 48 of static exposure to test substance up to 10 mg/L (nominal concentrations in agreement with measured concentration). The results showed that the 48-hour EC50 value was 7.1 mg a.i./L (nominal concentration).