5-ethylidene-8,9,10-trinorborn-2-ene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Comparable to guideline study. Well documented study which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Comparative acute toxicity and primary irritancy of the ethylidene and vinyl isomers of norbornene.

Author:

Ballantyne B, Myers R and Klonne D.

Year:

1997

Bibliographic source:

J. Applied Toxicology 17, 211-221.

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Principles of method if other than guideline:

Method not specified

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

5-ethylidene-8,9,10-trinorborn-2-ene

EC Number:

240-347-7

EC Name:

5-ethylidene-8,9,10-trinorborn-2-ene

Cas Number:

16219-75-3

Molecular formula:

C9H12

IUPAC Name:

5-ethylidenebicyclo[2.2.1]hept-2-ene

Details on test material:

- Test substance: 5-Ethylidene-2-norbornene (CASNo.16219-75-3)
- Purity: >99% as measured flame ionization gas chromatography.
- Source: Union Carbide Corporation, Institute, West Virginia.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: between 194-262 grams (males) and 137-206 grams (females)

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

2206, 2813, or 3431 ppm of ENB vapor in air

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 and 14 days

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LC50

Effect level:

2 717 ppm

95% CL:

2 318 - 3 184

Exp. duration:

4 h

Effect levels 2

Sex:

female

Dose descriptor:

LC50

Effect level:

3 015 ppm

95% CL:

2 404 - 3 780

Exp. duration:

4 h

Mortality:

7 of 10 rats died during exposure to 3431 ppm ENB.

Clinical signs:

other: All 7 rats that died during the period after exposure to ENB developed hindlimb parsalysis, and also showed urogenital area wetness, hypothermia, and absent surface righting and toe and tail pinch reflexes.

Body weight:

Body weights were unaffected for all survivors.

Gross pathology:

No gross pathology was seen in survivors at nescropsy.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The 4-hr LC50 value indicates a moderate degree of vapor toxicity. At these high vapor concentration, signs are predominantly those of a central excitatory nature, particularly hypereactivity, tremors, and convulsions.

Executive summary:

In a reliable study, Ballantyne et al (1997) published acute 4 -hr LC50 values of 2717 ppm (13.5 mg/l) (males) and 3015 ppm (15.1 mg/l) (females) for ENB vapor in rats.