2,2'-dimethyl-2,2'-azodipropiononitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Study period:

11/11/1983 - 01/20/1984

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Significant metholdological deficiences.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Groups of 10 male and 10 female rats were exposed to dust atmospheres of VazoR 64 for single 1-hour periods. The purpose of this study was to determine the 1-hour LC50 for VazoR 64 in male and female rats.

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

propanenitrile, 2,2'-azobis(2-methyl)-

IUPAC Name:

propanenitrile, 2,2'-azobis(2-methyl)-

Constituent 2

Reference substance name:

2,2'-dimethyl-2,2'-azodipropiononitrile

EC Number:

201-132-3

EC Name:

2,2'-dimethyl-2,2'-azodipropiononitrile

Cas Number:

78-67-1

Molecular formula:

C8H12N4

IUPAC Name:

2,2'-dimethyl-2,2'-azodipropiononitrile

Details on test material:

Purity > 98%

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding laboratories, Kingston, NY
- Age at study initiation: 8-9 week old
- Weight at study initiation: 240-265 g (males), 161-181g (females)
- Fasting period before study: no data
- Housing: in pairs (sexes separate) in 8''x8''x14'' stainless steel, wire-mesh cages.
- Diet (e.g. ad libitum): Purina certified rodent chow #5002, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period:n 1 week

ENVIRONMENTAL CONDITIONS
- Temperature : 74+/-2°F
- Humidity (%): 50+/-10
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: dust

Type of inhalation exposure:

nose only

Vehicle:

air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

1 h

Concentrations:

1.57 , 3.40, 7.78 mg/L

No. of animals per sex per dose:

10 animals/ sexe/dose

Control animals:

no

Details on study design:

All rats were weighed and observed daily for 2 weeks post exposure, except for the saturday and sunday of the second week post exposure.

Statistics:

no data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 7.78 mg/L air

Exp. duration:

1 h

Mortality:

One male rat died one day after exposure to 1.57 mg/l of Vazo 64. No death occurred after exposure followed by a return to a normal weight gain rate.

Clinical signs:

other: About half of the rats showed wet or stained perineal areas for one to two days after exposure.

Body weight:

Most rats showed moderate to severe weight losses one or two days after exposure followed by a return to a normal weight gain rate.

Applicant's summary and conclusion

Conclusions:

LC50 was not determined with this study. During exposures, only 10% at most of the airborne dust was of respirable size (<10 µm aerodynamic diameter).