No IUPAC name is currently defined for Cashew (Anacardium occidentale) Nutshell Extract, Decarboxylated, Distilled (“Distilled Grade”).

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The study was conducted to Good Laboratory Practice.

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Water solubility:

0.305 mg/L

Temp.:

20 °C

pH:

6.1

Details on results:

PRELIMINARY TEST:
Estimated water solubility of 0.000937 g/l

DEFINITIVE TEST:
Mean concentration: 0.000305 g/l at 20 ± 0.5°C
Range: 0.000257 to 0.000379 g/l

Table 2: Mean peak areas of the standards and samples in the definitive test

Solution	Mean Area
Standard 10.9 mg/l	104.638
Standard 10.9 mg/l	103.084
Sample 1 A	36.098
Sample 1 B	36.024
Sample 2 A	24.737
Sample 2 B	24.237
Sample 3 A	26.502
Sample 3 B	26.778

Table 3: Water solubility of test material in definitive test

Sample Number	Time shaken at 30°C (hours)	Time equilibrated at 20°C (hours)	Concentration (g/l)	Solution pH
1	24	24	3.79 x10^-4	5.9
2	48	24	2.57 x10^-4	6.1
3	72	24	2.80 x10^-4	6.3

The linearity of the detector response in respect to the concentration was assessed over the nominal concentration range of 0 to 15 mg/l. The response was satisfactory, with a correlation coefficient of 0.998 obtained. Recovery of analysis of the sample procedure was assessed and was adequate for the test.

Table 4: Recovery data

Nominal concentration (mg/l)	Recovery range (%)	Mean recovery (%)
1	86.8 to 113	99.4
0.2	99.0 to 128	111

Concentrations are not corrected for recovery analysis.

Conclusions:

Interpretation of results (migrated information): slightly soluble (0.1-100 mg/L)
The water solubility of Cashew (Anacardium occidentale) Nutshell Extract, Decarboxylated, Distilled (Distilled Grade) was determined to be 0.000305 g/l (0.305 mg/l) at 20 ± 0.5°C in an OECD Guideline 105 study, conducted under GLP conditions.

Executive summary:

The water solubility of Cashew Nutshell Extract, Decarboxylated, Distilled (Distilled Grade) liquid was determined in an OECD 105 Water Solubility (flask method) test. This study was conducted to Good Laboratory Practice.

A preliminary test was conducted with an aliquot of test material (0.539 g) diluted to 100 ml with glass double-distilled water. After shaking for 4 hours and standing at 20°C for 19 hours, the solution was centrifuged at 13500 rpm for 20 minutes prior to analysis. The water solubility was estimated to be 9.37 x10^-4 g/l.

In the definitive test, mixtures of test material and glass double-distilled water were added to three seperate flasks and shaken at 30°C. Flasks were left to stand at 20°C for a period of not less than 24 hours, then centrifuged at 10000 g for 30 minutes and sampled using pipettes. The pH of each solution was measured. Two 100 ml aliquots were taken per sample and extracted with three portions of dichloromethane and filtered through anhydrous sodium sulphate. The combined extracts were evaporated to dryness and the residue re-dissolved in 10 ml acetone.

The water solubility of the test material was determined to be 3.05 x10^-4 g/l at 20 ± 0.5°C.

This study is classified acceptable and satisfies the guideline requirement for a water solubility study.

Description of key information

The water solubility of Cashew Nutshell Extract, Decarboxylated, Distilled (Distilled Grade) was determined in an OECD 105 Water Solubility (Flask method) test. This study was conducted to Good Laboratory Practice. The water solubility of the test material was determined to be 0.305 mg/l at 20 ± 0.5°C. This study is classified acceptable and satisfies the guideline requirement for a water solubility study.

Key value for chemical safety assessment

Water solubility:

0.305 mg/L

at the temperature of:

20 °C