2-nitrotoluene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

other: EU Risk Assessment

Adequacy of study:

other information

Reliability:

other: EU Risk Assessment

Rationale for reliability incl. deficiencies:

other: no reliability is given as this is a summary entry for the EU RAR

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

European Union Risk Assessment Report - 2-Nitrotoluene

Author:

European Commission - European Chemicals Bureau

Year:

2008

Bibliographic source:

Office for Official Publications of the European Communities

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

1975

Report date:

1975

Reference 3

Reference Type:

publication

Title:

Toxic Hazards Evaluation of Five Atmospheric Pollutants from Army Ammunition Plants

Author:

Kinkead ER, MacEwen JD, Haun CC, Vernot EH and Dacre JC

Year:

1977

Bibliographic source:

National Technical Information Service. NTIS AD-A043 957

Reference 4

Reference Type:

other company data

Title:

Unnamed

Report date:

1972

Materials and methods

Principles of method if other than guideline:

EU Risk Assessment

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-nitrotoluene

EC Number:

201-853-3

EC Name:

2-nitrotoluene

Cas Number:

88-72-2

Molecular formula:

C7H7NO2

IUPAC Name:

1-methyl-2-nitrobenzene

Results and discussion

Any other information on results incl. tables

EU Risk Assessment (2008):

 

The acute dermal toxicity of 2-nitrotoluene has been investigated in two studies in rabbits and in one rat study. The studies were reported over the period 1972-77, pre-guideline performed and GLP non compliant. However, they appear to be broadly similar to modern test guidelines although the purity of the test substance was not reported.

 

Rats

A limit test was carried out at one dose level, 5000 mg/kgb.w. in a group of 6 female SPF Wistar rats. The test substance was applied undiluted on the clipped back for 24 hours, covered by a plaster and then washed, not observing either mortality or toxicity within 14-day observation period (Hollander and Weigand, 1975).

 

Rabbits

A limit test was carried out at one dose level, 20000 mg/kgb.w. in a group of 3 female albino rabbits. The test substance was applied undiluted to the clipped back and was kept in place by gauze patches, latex rubber, dental dam and elastic adhesive tape for 24 hours, and then removed. All rabbits were symptom free and gained weight normally during the subsequent 14-day observation period.

2-nitrotoluene was applied to the intact clipped on the dorsal skin of 6 male albino rabbits at a dose level of 200 mg/kgb.w. The rabbits´ trunks were then wrapped with a layer of Saran®

Wrap, stretch gauze bandage and elastic adhesive tape. After 24 hours, the wrappings were removed and the skin washed and dried. The animals were fitted with plastic collars to prevent ingestion of any residue and were observed for a further 48 hours. There were not mortality and clinical signs (McDonnell and Reinhardt, 1972).

 

Table 1: Summary of acute dermal toxicity of 2-nitrotoluene in experimental animals

Species	Dosage	LD 50 (mg/kgb.w.)	Comments	References
Rat, SPFWistar (female) 6rats/group	5000 mg/kg bw (24 h)	>5000 mg/kg bw	Purity not given Limit test	Hollander and Weigand (1975)
Rabbit,albino(male) 6rabbits/group	200 mg/kg bw (24 h)	>200 mg/kg bw	Purity not given	McDonnell and Reinhardt (1972)
Rabbit, albino New Zealand (female) 3 rabbits/group	20000 mg/kg bw.(24 h)	>20000 mg/kg bw	Purity not given Limit test	Kinkead et al. (1977)