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EC number: 210-765-4 | CAS number: 623-03-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
4 -Chlorobenzonitrile was not skin sensitising in an in vivo skin sensitisation test.
In a study according to OECD 429 ("Skin Sensitisation: Local Lymph Node Assay") 4-Chlorobenzonitrile formulated in DMF was assessed for its possible skin sensitising potential. The test was performed in mice using test item concentrations of 10, 25, and 50 % (w/w). The animals did not show any signs of systemic toxicity or local skin irritation during the course of the study and no cases of motality were observed. Stimulation Indices (S.I.) of 0.73, 0.51, and 0.72 were determined with the test item at concentrations of 10, 25, and 50 % (w/w) in DMF, respectively.
Therefore the test item 4-Chlorobenzonitrile wasnot a skin sensitiserunder the test conditions of this study.
Migrated from Short description of key information:
The dermal sensitisation potential of 4-Chlorobenzonitrile was
assessed in accordance with OECD guideline 429 ("Skin Sensitisation:
Local Lymph Node Assay". 4-Chlorobenzonitrile did not show any
sensitising potential in this study.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05.02.2014 - 18.02.2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
- Species:
- mouse
- Strain:
- other: CBA/CaOlaHsd
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan Laboratories B.V., Postbus 6174, 5960 AD Horst, The Netherlands
- Age at study initiation:9-10 weeks (at beginning of treatment)
- Weight at study initiation: 19.7 - 23.7 g
- Housing: group housing, cage type: Makrolon Type II (pre-test) / III (main study), with wire mesh top.
- Diet (e.g. ad libitum): 2018C Teklad Global 18 % protein rodent diet (certified), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: 15 days prior to the start of dosing under test conditions after health examination. Only animals without any visible signs of illness were used for the study.
ENVIRONMENTAL CONDITIONS
- Temperature (°C):22 +/- 2 °C
- Humidity (%): appox. 45-65 % (except for few hours on 5 days, see deviation)
- Photoperiod (hrs dark / hrs light): artificial light 6.00 a.m. - 6.00 p.m. - Vehicle:
- dimethylformamide
- Concentration:
- Pre-Test: 25, 50 % (w/w)
Main-Test:0, 10, 25, 50 % (w/w) - No. of animals per dose:
- Pre-Test: one animal per dose
Main-Test: four animals per dose - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- Statistics:
- The mean values and standard deviations of the body weights were calculated.
- Positive control results:
- Last 10 positive control experiments see table 2 in "any other information on results incl. tables".
- Key result
- Parameter:
- SI
- Value:
- >= 0.51
- Remarks on result:
- not determinable
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: see "any other information on results incl. tables"
- Key result
- Parameter:
- SI
- Value:
- >= 0.72
- Remarks on result:
- not determinable
- Key result
- Parameter:
- SI
- Value:
- >= 0.73
- Remarks on result:
- not determinable
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test item 4-Chlorobenzonitrile was not a skin sensitiser under the test conditions of this study accroding to OECD 429 ("Skin Sensitisation: Local Lymph Node Assay").
- Executive summary:
In a study according to OECD 429 ("Skin Sensitisation: Local Lymph Node Assay") 4-Chlorobenzonitrile formulated in DMF was assessed for its possible skin sensitising potential. The test was performed in mice using test item concentrations of 10, 25, and 50 % (w/w). The animals did not show any signs of systemic toxicity or local skin irritation during the course of the study and no cases of motality were observed. Stimulation Indices (S.I.) of 0.73, 0.51, and 0.72 were determined with the test item at concentrations of 10, 25, and 50 % (w/w) in DMF, respectively.
Therefore the test item 4-Chlorobenzonitrile was not a skin sensitiser under the test conditions of this study.
Reference
1. Calculation and Results of Individual Data
Table 1: Calculation and results of individual data.
Test item concentration % |
Group |
Measurement DPM |
Calculation |
Result |
||
DPM-BGa)[LM1] |
number of lymph nodes |
DPM per lymph nodeb) |
S.I. |
|||
--- |
BG I |
273 |
--- |
--- |
--- |
--- |
--- |
BG II[LM2] |
20 |
--- |
--- |
--- |
--- |
0 |
1 |
8083 |
7936.5 |
8 |
992.1 |
1.00 |
10 |
2 |
5957 |
5810.5 |
8 |
726.3 |
0.73 |
25 |
3 |
4219 |
4072.5 |
8 |
509.1 |
0.51 |
50 |
4 |
5829 |
5682.5 |
8 |
710.3 |
0.72 |
BG = Background; 1 = Control Group; 2-4= Test Group;
a) = The mean value was taken from the figures BG I and BG II;
b) = Since the lymph nodes of the animals of a dose group were pooled, DPM/node was determined by dividing the measured value by the number of lymph nodes pooled
The EC3 value could not be calculated, since all S.I. values are below the threshold value of 3.
2. Viability / Mortality
No deaths occurred during the study period.
3. Clinical Signs
No symptoms of local skin irritation at the ears of the animals and no signs of systemic toxicity were observed during the study period.
4. Body Weights
The body weight of the animals, recorded prior to the first application and prior to treatment with3HTdR, was within the range commonly recorded for animals of this strain and age.
5. Positive Control Data
Table 2: The last 10 positive control experiments.
Positive Control Substance |
Date |
Concentration / Vehicle |
S.I. value |
α-Hexylcinnamaldehyde |
October 2013 |
25% in acetone:olive oil (4+1, v/v) |
5.8 |
April 2013 |
5.9 |
||
October 2012 |
5.7 |
||
April 2012 |
3.7 |
||
February 2012 |
4.7 |
||
January 2012 |
10.8 |
||
January 2012 |
7.1 |
||
December 2011 |
5.9 |
||
November 2011 |
5.3 |
||
October 2011 |
6.1 |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
The dermal sensitisation potential of 4-Chlorobenzonitrile was assessed in accordance with OECD guideline 429 ("Skin Sensitisation: Local Lymph Node Assay". 4-Chlorobenzonitrile did not show any sensitising potential in this study. Therefore the substance does not need to be classified as skin sensitising substance.
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