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EC number: 233-828-8 | CAS number: 10377-66-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Study not to standard test method or to GLP, but sufficient detail available to allow assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Metal Bioaccumulation Patterns in Major Carps during Acute Toxicity Tests
- Author:
- ABDULLAH, S et al
- Year:
- 2 011
- Bibliographic source:
- International Journal of Agriculture & Biology;Oct2011, Vol. 13 Issue 5, p756
Materials and methods
- Principles of method if other than guideline:
- juvenile fish are exposed for specified period of time e.g., 96-h to a range of toxicant concentrations in a static system.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- manganese chloride terahydrate
- IUPAC Name:
- manganese chloride terahydrate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the end of each test, water samples were taken and analyzed for the corresponding metal concentration by following the methods as described in S.M.E.W.W. (1989). The analytical data obtained confirmed that the determined concentrations in the test mediums coincided with the estimated data quite satisfactorily. The concentrations of each metal in the fish (whole body) before and after acute toxicity tests were determined (S.M.E.W.W., 1989).
Test organisms
- Test organisms (species):
- other: Catla catla, Labeo rohita and Cirrhina mrigala
- Details on test organisms:
- TEST ORGANISM
- Source:Fish stock (induced bred) of desired weight and age were obtained from the Fish Seed Hatchery, Faisalabad, Pakistan.
- Age: 30 days, 60 days and 90 days
- Food and feeding: Fish were fed with crumbled feed (35% digestible protein & 2.90 kcal g-1 digestible energy) during adaptations, but they were not fed during the last 24 h of adaptations and throughout the test duration.
ACCLIMATION
- Acclimation period: 14 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 100 mg/L.
To maintain the total hardness, CaSO4 and MgSO4 were used to to increase hardness, while ethylenediaminetetraacetic acid and its sodium salts
(EDTA) were used to decrease water hardness when required. - Test temperature:
- 30oC
- Dissolved oxygen:
- During all the trials, constant air was supplied to the test mediums with air pumped through a capillary system.
- Details on test conditions:
- TEST SYSTEM
All glassware and aquariums used in this experiment were washed with nitric acid and thoroughly rinsed with deionized water prior to use.
Prior to each trial, all aquariums (70-L capacity) were filled with 50-L dechlorinated tap water of desired hardness (100 mg L-1) and pH (7).
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
:
OTHER TEST CONDITIONS
- Adjustment of pH: NaOH and HCl were added as required to maintain pH
- Photoperiod: 12 hours
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dead fish were weighed individually after being lightly blotted dry at the time of mortality observations. No mortality was observed among control fish.
TEST CONCENTRATIONS
The concentrations tested for three fish species, separately, were started from zero with an increment of 0.05 and 5 mg L-1 (as total concentration) for low and high concentrations, respectively.
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 55.26 - <= 67.71 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- mortality (fish)
- Reported statistics and error estimates:
- Statistical analyses were performed with SPSS 10.1 Computer program. Differences in metal toxicity means among species were analyzed by Analysis of Variance and Duncan’s Multiple Range tests (Steel et al., 1996). Relationships among various parameters were determined from Pearson Correlation Coefficients.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mean LC50 and lethal concentrations of three fish age groups
Fish Species
LC50 concentrations (±SE) (mgL-1)
Lethal concentrations (±SE) (mgL-1)
30-day
60-day
90-day
30-day
60-day
90-day
Catla catla
55.26 ± 3.67
64.67 ± 3.61
67.71 ± 3.94
92.03 ± 7.20
99.59 ± 6.52
107.72±6.83b
Labeo rohita
64.13 ± 3.39
71.15 ± 3.77
73.70 ± 3.64
99.82 ± 6.63
110.54 ±7.04
108.74±6.44
Cirrhina mrigala
71.24 ± 3.87
76.23 ± 4.55
91.68 ± 4.65
110.43± 6.81
123.21± 8.11
142.13±9.66
Mean metals concentrations in three age groups of fish before and after acute exposure
30-day
60-day
90-day
Before exposure
After exposure
Before exposure
After exposure
Before exposure
After exposure
Catla catla
8.19 ± 0.21
61.61 ± 2.32
10.39±0.43
158.79± 11.11
11.11 ± 0.23
160.12 ± 1.23
Labeo rohita
8.62 ± 0.36
67.39 ± 3.99
11.65±0.52
168.42±42.43
11.69 ± 0.58
168.88 ± 2.52
Cirrhina mrigala
8.73 ± 0.69
79.15 ± 1.79
12.84±0.63
183.39±23.46
13.15 ± 0.36
186.15 ± 1.95
Applicant's summary and conclusion
- Conclusions:
- The 96 h LC50 for Catla catla (Indian carp) was reported as 55-68 mg/L
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