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Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Febuary 11,2013 - March 11, 2013
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
according to guideline
other: ISO standard 7346/3
GLP compliance:
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Physico-chemical properties
- water solubility:2.59 - 2.96 g/l
Analytical monitoring:
Details on sampling:
In order to verify the actual concentration of the test product in the test solutions, samples were collected at test start, before and after renewal af the test solutions and at the end of the test for chemical analysis of the test product. Duplicate samples of 10 ml were collected in 25 ml plastic vials. The samples were stored at -20 ± 2.0°C . Only one of these samples were analysed and only those relevant for calculation of the LCx concentrations. The spare samples were discarded after sponsor approved the report.
Details on test solutions:
A stock solution of 120 mg/l were prepared by weighing out 240 mg of the test product and dissolving it in 2 l of zebra fish test medium. The test solution were prepared by diluting the stock solution in the zebra fish test medium. A fresh stock solution were prepared for renewal for the test solution after 48 hours.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
The test fish were obtained from Credo Fish, Nørresundby, Denmark
The fish had a length of 2±1 cm
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Nominal and measured concentrations:
The analytically determined test item concentrations in the analyzed test media samples at the start of the test.
The values found at the end of the test ranged from 90 to 104% of the nominel values. Under the test conditions the test item was stable during the test period of 96 hours.
Key result
96 h
Dose descriptor:
Effect conc.:
63 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Validity criteria fulfilled:
The 96 h LC50 to zebra fish amounted to 63 mg/l.
The validation of the test concentrations by chemicals analyses showed a deviation from the nominel concentration throughout the test lower than 20 per cent. Therefore results are based on the nominel concentration.

Description of key information

Hazardous to the aquatioc environment. Chronic Category 3

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
63 mg/L

Additional information

A study conducted according to OECD guideline 203 is available. Zebra fish were used as test organisms and the LC50 after 96 hours of exposure was determined to be 63 mg/L. The results were based on the nominal measured test item concentrations.