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EC number: 603-491-5 | CAS number: 131513-00-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
In an algae growth inhibition test the EC50 was 0.261 mg/L after 72 h, the EC20 was 0.152 mg/L after 72 h and the EC10 was 0.113 mg/L after 72 hours. The values are based on geometric mean measured test test item concentrations of the inhibition of growth rate.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.261 mg/L
- EC10 or NOEC for freshwater algae:
- 0.113 mg/L
Additional information
The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 and Council Regulation (EC) No 440/2008/ Method C.3. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours. The study was conducted under static conditions with an initial cell density of 4910 cells/mL. Based on a preliminary test, 5 concentration Ievels were tested in a geometrical series with these nominal concentrations: 0.1 - 0.32 - 1.0 - 3.2 - 10 mg/L, corresponding to geometric mean measured concentrations of 0.0440 - 0.0716 - 0.126 - 0.220 - 0.408 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. Environmental conditions were determined to be within the acceptable Iimits. The concentrations of the test item were analytically verified by HPLCDAD at the start and the end of the exposure of all test concentrations and the control. The measured concentrations of the test item at the start of the exposure were in the range of 60 to 78% of the nominal values and < Iimit of quantification after 72 hours. Therefore, all effect values are based on both the nominal and the geometric mean measured concentrations of the test item. Instead of the test item, another compound with a relative retention time of 0.36 (absorption maximum 291 nm) was detected in all exposure concentrations after 72 hours. This peak was integrated. These data show that the magnitude of the exposure concentrations are also reflected by the magnitude of the areas of the metabolite. The EC50 was 0.261 after 72 h, the EC20 was 0.152 after 72 h and the EC10 was 0.113 mg/L after 72 hours. The values are based on geometric mean measured test item concentrations of the inhibition of growth rate.
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