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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Within 28 days a degradation rate of 3 % was determined. The test item is considered to be "Not Readily Biodegradable". The reference compound anilline showed 72 % degradation after 14 days.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The biodegradtion of the substance was determined according to EU method C.4 -D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test). The substance was incubated for 28 days in contiuously stirred 250 mL closed flask (in duplicate) in the dark with an inoculum originally collected from a local, predominantly municipal wastewater treatment plant. In this assay, biodegradation was estimated by biological oxygen demand (BOD) over time. BOD was measured daily by a manometer. The inoculum temperature was 22 °C. The concentration of inoculum was 30 mg/L and the one of the substance was 100 mg/L. Degradation was calculated by substracting the amount BOD in the negative (inoculum only) control from that in the substance or positive control at any given time point and divided by chemical oxygen demand (COD) or thereotical oxygen demand (ThOD). The 28 -day degradation was 3 % and for the positive control (with reference substance) the biodegradation was 82 %. In conclusion the substance is "Not Readily Biodegradable".