2-fluoro-6-trifluoromethylpyridine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

428-100-3

EC Name:

-

Cas Number:

94239-04-0

Molecular formula:

C6H3NF4

IUPAC Name:

2-fluoro-6-(trifluoromethyl)pyridine

Details on test material:

- Purity: 99.56%

Specific details on test material used for the study:

Substance ID: F6TF
Purity: 99.56%

Test animals

Species:

rat

Strain:

other: Sprague Dawley(SD), Grade SPF

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 8 weeks
- Weight at study initiation: male rats 202-216 g, female rats 186-192 g
- Housing: housed one rat per cage in suspended stainless steel wire mesh cages
- Diet (e.g. ad libitum): amount not reported
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-22ºC
- Humidity (%): 50-52%

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: Soybean oil

Details on dermal exposure:

TEST SITE
- Area of exposure: test substance was applied uniformly over an area which was approximately 10% of the total body surface area
- Type of wrap if used: held in contact with the skin with a porous gauze dressing and non-irritating tape

REMOVAL OF TEST SUBSTANCE
- Washing: yes, warm water
- Time after start of exposure: 24 hours

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2500 mg/kg body weight

Duration of exposure:

24 hours

Doses:

2500 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily after test substance administration and at sacrifice
- Frequency of weighing: shortly before test substance administration, weekly thereafter, and at sacrifice
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 500 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed.

Clinical signs:

other: No clinical signs were observed.

Gross pathology:

No abnormalities were found during necropsy.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Rat Dermal LD50: >2500 mg/kg

Executive summary:

Male and female Sprague Dawley rats were dosed with the test substance in a dermal limit test according to the State Environmental Protection Administration's Guidelines for Testing of Chemicals (402) at a dose of 2500 mg/kg.  There were no obvious signs of toxicity and no mortality occurred during the study. No abnormalities were found during necropsy.  According to these results, an approximate acute dermal LD₅₀ for the test substance in rats is >2500 mg/kg for male and female rats.