2-fluoro-6-trifluoromethylpyridine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

The fish were held for 6 days at approximately 15°C before the test. This is less than the guideline requirement of being on site for 12 days and acclimatised for at least 7 days.

GLP compliance:

yes

Specific details on test material used for the study:

Substance ID: F6TF
Lot #: AF0460
Purity: 99.5%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all
- Sampling method: high performance liquid chromatography; Samples were taken from the centre of the test solution.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances): The test solution was prepared by the direct addition of the required amount of the test substance to the exposure vessel. The test solution was then stirred thoroughly for 24 hours to allow complete dissolution of the test substance. The test solutions were observed to be clear and colourless. The test solution was not changed or aerated during the 96 hour exposure period.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Houghton Springs Fish Farm, Winterboume Houghton, Blandford Forum, Dorset, UK
- Length at study end: 35 - 41 mm; mean 38 mm
- Weight at study end: 0.54 - 1.24 g; mean 0.76 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: 6 days
- Acclimation conditions (same as test or not): yes; 15°C
- Type and amount of food: Keystart 1200® (FF417)
- Health during acclimation (any mortality observed): 1 dead

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

42.3 mg/L as CaC03

Test temperature:

15 ± 1°C

pH:

6.98 - 7.89

Dissolved oxygen:

3.6 - 10.0 mg/L

Salinity:

freshwater

Nominal and measured concentrations:

Nominal: 0, 10, 18, 32, 56 and 120 mg/L
Measured: 0, 11, 19, 34, 58 and 110 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: spherical glass vessels of 36 cm external diameter, with a nominal capacity of 20 L, fitted with Quickfit glass® lids and stoppers (sealable due to volatile test substance)
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Observations for mortalities and symptoms of toxicity were made at 24, 48, 72 and 96 hours.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

82 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 56 - 120 mg/L

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: symptoms of toxicity

Details on results:

The mean measured values of the test solutions ranged from 100 to 106% of the nominal values.

Dissolved oxygen values ranged from 3.6 to 10.0 mg/L. No mortalities occurred in the low DO test vessels.

The fish in the 120 mg/L test solution were terminated at 3.75 hours. Their symptoms were loss of balance, dark discoloration and laboured breathing.

Reported statistics and error estimates:

The LC50 values, and their respective 95% confidence intervals, were calculated at various time intervals by the testing facility computer program "LC50" using Stephan's method

Sublethal observations / clinical signs:

Observed Symptoms of Toxicity

Nominal Concentration  (mg/L)	24 hour	48 hour	72 hour	96 hour
10	A	A	A	A
18	A	A	A	A
32	A	A	A	A
56	Cabc	Cabc	Cabc	Cc
120	TD*	TD	TD	TD

A = 10% or less of test population exhibiting symptoms of toxicity (considered not to be biologically significant).

B = Between 11 % and 30% of test population exhibiting symptoms of toxicity.

C = More than 30% of test population exhibiting symptoms of toxicity.

TD* = Terminated after 3.75 hours on day 0

a = Quiescent

b = Surfacing

c = Dark discoloured

 

Validity criteria fulfilled:

yes

Conclusions:

96-h LC50 (Rainbow Trout) = 82 mg/L

Executive summary:

An acute static 96-hour test was conducted at concentrations of 0, 10, 18, 32, 56 and 120 mg/L on rainbow trout according to the guideline OECD 203. The test was run in sealed vessels because of the volatility of the test substance. After 3.75 hours the test at 120 mg/L was terminated due to the toxic effects on the fish. Based on nominal concentration, the 96-hour LC₅₀ was 82 mg/L.

Description of key information

Based on nominal concentrations because mean measured concentrations were within 80-120% of nominal:
96-hour LC50 = 82 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

82 mg/L