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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Not readily biodegradable (29 % degradation after 28 days); OECD 301 D, EU Method C.6; Douglas and Halls (1992)

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The ready biodegradability of the test material was determined in accordance with the standardised guidelines OCED 301 D and EU Method C.6 by the measurement of dissolved oxygen, and Chemical Oxygen Demand (COD), in a closed bottle test. Under the conditions of the study, the test material attained only 29% biodegradation after 28 days and cannot, therefore, be termed as readily biodegradable. Suitable biodegradation was observed with the reference substances used. After 28 days the oxygen depletion value for the inhibition check culture (g) was 83% of the sum of the individual component substances i.e. test material on filter paper plus sodium benzoate. This indicates that no significant inhibition occurred at the test concentration of 2 mg/L. Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits.

The study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting. The study was assigned a reliability score of 1 and considered suitable for assessment as an accurate reflection of the test material. The available data are considered to be complete and the conclusion, not readily biodegradable, was taken forward for risk assessment.