Registration Dossier
Registration Dossier
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EC number: 229-654-7 | CAS number: 6640-24-0
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-(m-chlorophenyl)piperazine
- EC Number:
- 229-654-7
- EC Name:
- 1-(m-chlorophenyl)piperazine
- Cas Number:
- 6640-24-0
- Molecular formula:
- C10H13ClN2
- IUPAC Name:
- 1-(3-chlorophenyl)piperazine
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at the highest dose-level in the absence of S9 in TA1535, TA1537,TA100, TA102.
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test substance did not induce two fold increases in the number of revertant colonies at any dose-level, either in the absence or presence of S9 metabolism.
Toxicity as indicated by thinning of the background lawn, was observed at the highest dose-level in the absence of S9 metabolism in all tester strains with the exception of TA1538 and TA98. Tester strains TA100 and TA102 appeared to be more susceptible to the toxic effect of the test substance and reductions in revertant numbers, thinning of the background lawn and/or microcolony formation were observed at higher dose-levels, in the absence and presence of S9 metabolism.
Positive control treatments produced marked increases in revertant numbers, indicating the correct functioning of the assay system.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test substance does not induce reverse mutation in Salmonella typhimurium with and without metabolic activation, under the reported experimental conditions.
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