1,4-bis(p-tolylamino)anthraquinone

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian cell study: DNA damage and/or repair

Remarks:

Sister chromatid exchange (SCE)

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Reference 2

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1987

Materials and methods

Principles of method if other than guideline:

In vivo sister chromatid exchange in male C57BL./6 mouse bone marrow cells. Solvent Yellow 33/Solvent Green 3 mixture at doses of 10, 20, or 40 mg/kg in 0.1 mL DMSO + 0.1 mL corn oil was injected intraperitoneally (3 to 4 animals/group). Positive controls were injected with cyclophosphamide, and negative controls were injected with the vehicle.

GLP compliance:

no

Type of assay:

sister chromatid exchange assay

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

Cas Number:

8003-22-3

Constituent 2

Reference substance name:

1,4-bis(p-tolylamino)anthraquinone

EC Number:

204-909-5

EC Name:

1,4-bis(p-tolylamino)anthraquinone

Cas Number:

128-80-3

Molecular formula:

C28H22N2O2

IUPAC Name:

1,4-bis[(4-methylphenyl)amino]-9,10-anthraquinone

Test animals

Species:

mouse

Strain:

C57BL

Details on species / strain selection:

C57BL/6

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

0.1 ml DMSO + 0.1 ml corn oil

Duration of treatment / exposure:

Single administration.

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 10, 20 and 40 mg/kg
Basis: Solvent Yellow 33/Solvent Green 3 mixture

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Remarks on result:

other:

Remarks:

None of the doses was effective in inducitg sister chromatAd exchange in bone marrow cells.

Applicant's summary and conclusion

Conclusions:

Interpretation of results: negative

Executive summary:

In vivo sister chromatid exchange in male C57BL/6 mouse bone marrow cells was analyzed as another test for genotoxicity (Moore et al, 1984). Solvent Yellow 33/Solvent Green 3 mixture at doses of 10, 20, or 40 mg/kg in 0.1 mL DMSO + 0.1 mL corn oil was injected intraperitoneally (3 to 4 animals/group). Positive controls were injected with cyclophosphamide, and negative controls were injected with the vehicle. The results showed that none of the doses was effective in inducing sister chromatid exchange in bone marrow cells. The dye was not cytotoxic; nevertheless, Moore ot

al. (1984) presumed that the dye was distributed to bone marrow cells, although they found no traces of the dye in bone marrow cells.