1,4-bis(p-tolylamino)anthraquinone

Endpoint summary

Description of key information

The purpose of the Local Lymph Node Assay was to identify the contact allergenic potential of the test chemical when administered to the dorsum of both ear lobes of mice. The study was performed according to OECD 429 and Commision Directive 2004/73/EC, B.42: Skin Sensitization: Local Lymph Node Assay, 29 April 2001 Guidelines.

Calculation of the EC 3 value was not done because no test concentrations produced a STIMULATION INDEX (S.I.) of 3 or higher. In this study STIMULATION INDICES of 1.8, 0.8 and 1.2 were determined with the test item at concentrations of 2.5 %, 5 % and 10 % (w/v) , respectively, in acetone/olive oil (4/1 (v/v). The test chemical was therefore found to be a non-sensitizer when tested at up to the highest applicable concentration of 10 % (w/v) in acetone/olive oil (4/1 (v/v).

A modified guinea pig testing technique, the adjuvant and patch test was employed to evaluate the contact hypersensitivity of test chemical on Hartley strain female albino guinea pigs.

The fractional response (FR) and mean response (MR) was observed to be 0.0 at each tested concentration.

Since no response was detected to purified test preparation (1, 0.1 and 0.01%) in test and control group, the chemical was considered to be not sensitizing to the skin of Hartley strain female albino guinea pigs.

Key value for chemical safety assessment

Skin sensitisation

Link to relevant study records

Reference

Reference 1

Endpoint:

skin sensitisation: in vivo (LLNA)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from experimental report

Qualifier:

according to guideline

Guideline:

OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)

Qualifier:

according to guideline

Guideline:

other: Commision Directive 2004/73/EC, B.42: Skin Sensitization: Local Lymph Node Assay, 29 April 2001.

Principles of method if other than guideline:

The purpose of the Local Lymph Node Assay was to identify the contact allergenic potential of the test chemical when administered to the dorsum of both ear lobes of mice.

GLP compliance:

yes

Type of study:

mouse local lymph node assay (LLNA)

Species:

mouse

Strain:

CBA

Remarks:

CBA/CaOlaHsd

Sex:

female

Details on test animals and environmental conditions:

TEST ANIMALS
- Source: Harlan Netherlands; B.V. Postbus 6174; NL - 5960 AD Horst / The Netherlands
- Females nulliparous and non-pregnant: yes
- Microbiological status of animals, when known: No data
- Age at study initiation: 8 - 12 weeks
- Weight at study initiation: 16 g - 24 g
- Identification: Each cage by unique cage card.
- Housing: Individual in Makrolon type-2 cages with standard softwood bedding ("Lignocel", Schill AG,CH-4132 Muttenz).
- Diet (e.g. ad libitum): Pelleted standard Kliba 3433, batch no. 92/04 mouse maintenance diet (Provimi Kliba AG, CH-4303 Kaiseraugst) available ad libitum.
- Water (e.g. ad libitum): Community tap water from Itingen, available ad libitum.
- Acclimation period: 6 days.
- Indication of any skin lesions: No data
- Acclimation period: 6 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3 degC
- Humidity (%): 30 - 70 %
- Air changes (per hr): 10 - 15 air changes per hour.
- Photoperiod (hrs dark / hrs light): 12 hour fluorescent light / 12 hour dark cycle with at least 8 hours music during the light period.

- IN-LIFE DATES: From:09-FEB-2005 To:23-FEB-2005

Vehicle:

acetone/olive oil (4:1 v/v)

Concentration:

1 (Control Group)
Group 2 - 2.5 % (w/v)
Group 3 - 5 % (w/v)
Group 4 - 10 % (w/v)

No. of animals per dose:

1 (Control Group) - 4 animals
Group 2 - 4 animals
Group 3 - 4 animals
Group 4 - 4 animals

Details on study design:

PRE-TEST
In a non-GLP solubility pre-test, the test item was tested in different vehicles: acetone/olive oil, (4/1, v/v), dimethylsulfoxide (DMSO) and ehanol/water (7/3, v/v).A suitable vehicle (acetone/olive oil, (4/1, v/v)) was selected and used in the main test.
In a non-GLP animal pre-test in two mice, the test item was tested at four different concentrations: 1 %,2.5 %, 5 % and 10 % (w/v) , on one ear each.
24 hours after a single topical application, the pre-test results determined that 10 % (w/v) was the highest technically applicable concentration while avoiding systemic toxicity and excessive local irritation in the chosen vehicle.

TREATMENT PROCEDURES
TOPICAL APPLICATION
Each test group of mice was treated by topical (epidermal) application to the dorsal surface of each ear lobe (Ieft and right) with different test item concentrations of 2.5 %, 5 % and 10 % (w/v) in acetone/olive oil (4/1 (v/v). The application volume, 25 μl, was spread over the entire dorsal surface (Ø - 8 mm) of each ear lobe once daily for three consecutive days. A further group of mice was treated with an equivalent volume of the relevant vehicle alone (control animals). A hair dryer was passed briefly over the ear's surface to prevent the loss of any of the test item applied.

ADMINISTRATION OF 3H-METHYL THYMIDINE*
3H-methyl thymidine (3HTdR) was purchased from Amersham International (Amersham product code no. TRA 310; specific activity, 2 Ci/mmol; concentration,1 mCi /ml).Five days after the first topical application, all mice were administered with 250 μI of 76.13 μCi/ml 3HTdR (equal to 19.0 μCi 3HTdR) by intravenous injection via a tail vein.

DETERMINATION OF INCORPORATED 3HTDR*
Approximately five hours after treatment with 3HTdR all mice were euthanized by inhalation of CO2 (dry ice).
The draining Iymph nodes were rapidly excised and pooled for each experimental group (8 nodes per group). Single cell suspensions (phosphate buffered saline) of pooled Iymph node cells were prepared by gentle mechanical disaggregation through stainless steel gauze (200 μm mesh size). After washing two
times with phosphate buffered saline (approx. 10 ml) the Iymph node cells were resuspended in 5 % trichloroacetic acid (approx. 3 ml) and incubated at approximately +4 °C for at least 18 hours for precipitation of macromolecules.The precipitates were then resuspended in 5 % trichloroacetic acid (1 ml) and transferred to glass scintillation vials with 10 ml of 'Irga-Safe Plus' scintillation liquid and thoroughly mixed.The level of 3HTdR incorporation was then measured on a ß-scintillation counter. Similarly, background 3HTdR levels were also measured in two 1 ml-aliquots of 5 % trichloroacetic acid.The ß-scintillation counter expresses 3HTdR incorporation as the number of radioactive disintegrations per minute (DPM).

Positive control substance(s):

hexyl cinnamic aldehyde (CAS No 101-86-0)

Statistics:

The mean values and standard deviations were calculated in the body weight tables.

Positive control results:

Positive control results
CALCULATION AND RESULTS OF INDIVIDUAL DATA
The proliferative capacity of pooled Iymph node cells was determined by the incorporation of 3H-methylthymidine measured on a ß-scintillation counter.
Test item concentration % (w/v) S.I.
Group 2 5* 2.4*
Group 3 10• 3.6 •
Group 4 25 11.2
EC3 = 7.5 % (w/v)
A clear dose-response relationship was observed.
• This value was used in calculation of EC3.

VIABILITY / MORTALITY
No deaths occurred during the study period.

CLINICAL SIGNS
No clinical signs were observed in any animals of the control group.On the second application day,a slight ear swelling was observed at both dosing sites in all mice of group 4(25%), persisting for the remainder of the in-life phase of the study.One day after the third local application, a slight ear swelling was observed at both dosing sites in all mice of Group2 (5%) and Group3 (10%), persisting for the remainder of the in-life phase of the study.

BODY WEIGHTS
The body wejght of the animals, recorded prior to the 1st application and prior to necropsy,was within the range commonly recorded for animals of this strain and age.

CONCLUSION
A test item is regarded as a sensitizer in the LLNA if the exposure to one or more test concentrations resulted in 3-fold or greater increase in incorporation of 3HTdR compared with concurrent controls, as indicated by the STIMULATION INDEX (S.I.).
In this study STIMULATION INDICES of 2.4, 3.6 and 11.2 were determined with the test item at concentrations of 5 %, 10 % and 25 % (w/v) , respectively, in acetone:olive oil, 4:1 (vIv).
ALPHA-HEXYLCINNAMALDEHYDE was therefore found to be a skin sensitizer and an EC3 value of 7.5 % (w/v) was derived.

Key result

Parameter:

SI

Value:

1.8

Test group / Remarks:

Group2: 2.5 % (w/v) in acetone/olive oil (4/1, v/v).

Remarks on result:

other: not sensitizing

Key result

Parameter:

SI

Value:

0.8

Test group / Remarks:

Group3: 5 % (w/v) in acetone/olive oil (4/1, v/v).

Remarks on result:

other: not sensitizing

Key result

Parameter:

SI

Value:

1.2

Test group / Remarks:

Group4: 10 % (w/v) in acetone/olive oil (4/1, v/v).

Remarks on result:

other: not sensitizing

Cellular proliferation data / Observations:

CELLULAR PROLIFERATION DATA
The proliferative capacity of pooled Iymph node cells was determined by the incorporation of 3H-methylthymidine measured on a ß-scintillation counter.
Test item concentration% (w/v) S.l.
Group 2 2.5 1.8
Group 3 5 0.8
Group 4 10 1.2
No dose-response relationship was observed.
Calculation of the EC3 value was not done because no test concentrations produced a STIMULATION INDEX (S.I.) of 3 or higher.

EC3 CALCULATION
Calculation of the EC3 value was not done because no test concentrations produced a STIMULATION INDEX (S.I.) of 3 or higher.

CLINICAL OBSERVATIONS:
No clinical signs were observed in any animals of the control group. After the first application, both ears of all test item groups of mice (Groups 2-4) showed black at dosing sites, persisting for the remainder of the in-life phase of the study.

BODY WEIGHTS
The body weight of the animals, recorded prior to the first application and prior to neroscopy,was within the range commonly recorded for animals of the strain and age.

VIABILITY / MORTALITY
No deaths occurred during the study period.

Positive Control Study

SUMMARY

In order to study a possible contact allergenic potential of ALPHAHEXYLCINNAMALDEHYDE,three groups each of four female mice were treated daily with the test item at concentrations of 5 %, 10 % and 25 %(w/v) in acetone:olive oil, 4: 1 (v/v) by topical application to the dorsum of each ear lobe (Ieft and right) for three consecutive days.

A control group of four mice was treated with the vehicle (acetone:olive oil, 4:1 (v/v)) only.

Five days after the first topical application the mice were injected intravenously into a tail vein with radio-Iabelled thymidine (3H-methyl thymidine). Approximately five hours after intravenous injection, the mice were sacrificed, the draining auricular lymph nodes excised and pooled per group. Single cell suspensions of lymph node cells were prepared from pooled lymph nodes which were subsequently washed and incubated with trichloroacetic acid overnight. The proliferative capacity of pooled Iymph node cells was determined by the incorporation of 3H-methyl thymidine measured in a ß-scintillation counter.

All treated animals survived the scheduled study period.

No clinical signs were observed in any animals of the control group.On the second application day,a slight ear swelling was observed at both dosing sites in all mice of group 4 (25%), persisting for the remainder of the in-life phase of the study.One day after the third local application, a slight ear swelling was observed at both dosing sites in all mice of Group2 (5%) and Group3 (10%), persisting for the remainder of the in-life phase of the study.

The results obtained (STIMULATION INDEX (S.I.)) are reported in the following table. The estimated concentration of test item required to produce a S.I. of 3 is referred to as the EC3 value.

	Test item concentration % (w/v)	S.I.
Group 2	5*	2.4*
Group 3	10*	3.6*
Group 4	25	11.2
EC3 = 7.5 % (w/v)
A clear dose-response relation was observed. • This value was used in calculation of EC3.

Interpretation of results:

other: Not sensitising

Conclusions:

A test item is regarded as a sensitizer in the LLNA if the exposure to one or more test concentrations resulted in 3-fold or greater increase in incorporation of 3HTdR compared with concurrent controls, as indicated by the STIMULATION INDEX (S.I.).
In this study STIMULATION INDICES of 1.8, 0.8 and 1.2 were determined with the test item at concentrations of 2.5 %, 5 % and 10 % (w/v) , respectively, in acetone/olive oil (4/1 (v/v).
The test chemical was therefore considered to be a non-sensitizer when tested at up to the highest applicable concentration of 10 % (w/v) in acetone/olive oil (4/1 (v/v).

Executive summary:

The purpose of the Local Lymph Node Assay was to identify the contact allergenic potential of the test chemical when administered to the dorsum of both ear lobes of mice. The study was performed according to OECD 429 and Commision Directive 2004/73/EC, B.42: Skin Sensitization: Local Lymph Node Assay, 29 April 2001 Guidelines.

In order to study a possible contact allergenic potential of the test chemical, three groups each of four female CBA/CaOlaHsd mice were treated daily with the test item at concentrations of 2.5 %, 5% and 10% (w/v) in acetone/olive oil (4/1, v/v) by topical application to the dorsum of each ear lobe (left and right) for three consecutive days. 10 % was the highest technically applicable concentration in the vehicle. A control group of four mice was treated with the vehicle (acetone/olive oil (4/1, v/v)) only. Five days after the first topical application the mice were injected intravenously into a tail vein with radio-labelled thymidine (3H-methyl thymidine). Approximately five hours after intravenous injection, the mice were sacrificed, the draining auricular lymph nodes excised and pooled per group. Single cell suspensions of lymph node cells were prepared from pooled lymph nodes which were subsequently washed and incubated with trichloroacetic acid overnight. The proliferative capacity of pooled lymph node cells was determined by the incorporation of 3H-methyl thymidine measured in a ß-scintillation counter. All treated animals survived the scheduled study period. No clinical signs were observed in any animals of the control group. After the first application, both ears of all test item groups of mice (Groups 2-4) showed black at dosing sites, persisting for the remainder of the in-life phase of the study. The results obtained (STIMULATION INDEX (S.I.)) are reported in the following table. Calculation of the EC 3 value was not done because no test concentrations produced a STIMULATION INDEX (S.I.) of 3 or higher.

In this study STIMULATION INDICES of 1.8, 0.8 and 1.2 were determined with the test item at concentrations of 2.5 %, 5 % and 10 % (w/v) , respectively, in acetone/olive oil (4/1 (v/v).

The test chemical was therefore found to be a non-sensitizer when tested at up to the highest applicable concentration of 10 % (w/v) in acetone/olive oil (4/1 (v/v).

Reference 2

Endpoint:

skin sensitisation: in vivo (non-LLNA)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data from publication

Qualifier:

according to guideline

Guideline:

other: the adjuvant and patch test

Principles of method if other than guideline:

A modified guinea pig testing technique, the adjuvant and patch test was conducted on Hartley strain female albino guinea pigs to evaluate the contact hypersensitivity of test chemical

GLP compliance:

not specified

Type of study:

patch test

Justification for non-LLNA method:

not specified

Species:

guinea pig

Strain:

Hartley

Sex:

female

Details on test animals and environmental conditions:

TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: 370 - 420 g
- Fasting period before study: No data
- Housing: The animals were housed singly in stainless steel wire-mesh cages
- Diet (e.g. ad libitum): A standard guinea pig diet, ad libitum.
- Water (e.g. ad libitum): Water, ad libitum
- Acclimation period: no data available

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23±2 ◦C
- Humidity (%): 55±5%
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): 12 h daily

IN-LIFE DATES: No data

Route:

intradermal

Vehicle:

other: Acetone

Concentration / amount:

Aliquots of 0.1 ml each of a water-in-oil type emulsion (distilled water: FCA =1:1) were injected intradermally into the four corners of a previously shaved shoulder region (2 cm×4 cm). At the injection sites, scratches in the shape of a grid were made with the needle used for injection.

Day(s)/duration:

not specified

Adequacy of induction:

not specified

Route:

epicutaneous, occlusive

Vehicle:

other: acetone

Concentration / amount:

0.1 ml of 1, 0.1 and 0.01%

Day(s)/duration:

24 hours

Adequacy of induction:

not specified

No.:

#1

Route:

epicutaneous, occlusive

Vehicle:

other: acetone

Concentration / amount:

0.1 ml of 1, 0.1 and 0.01%

Day(s)/duration:

48 hours

Adequacy of challenge:

not specified

No. of animals per dose:

20 (10 animals/ group)

Details on study design:

MAIN STUDY
A. INDUCTION EXPOSURE: intradermal
Aliquots of 0.1 ml each of a water-in-oil type emulsion (distilled water: FCA =1:1) were injected intradermally into the four corners of a previously shaved shoulder region (2 cm×4 cm). At the injection sites, scratches in the shape of a grid were made with the needle used for injection.

B. INDUCTION EXPOSURE: dermal
- No. of exposures: 2
- Exposure period: 24 hours
- Test groups: 10
- Control group: 10
- Site: no data
- Frequency of applications: no data
- Duration: 48 hours
- Concentrations: 0.1 ml of 1, 0.1 and 0.01%


B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: after 2 weeks
- Exposure period: 24 hours
- Test groups: 10
- Control group: 10
- Site: shaved skin of the back
- Concentrations: 0.1 ml of 1, 0.1 and 0.01%
- Evaluation (hr after challenge): after 24 days
OTHER: during topical induction, a closed patch with 0.1 ml of test preparation was applied to the sites for 24 h. Abrasions and sample applications were repeated on the following 2 days. One week after the initial sensitization, 10% sodium lauryl sulfate in petrolatum was applied to the intradermal injection sites. On the next day, a closed patch of the test preparation was applied at the same sites for 48 h.

Challenge controls:

yes, concurrent vehicle.

Positive control substance(s):

not specified

Key result

Reading:

1st reading

Hours after challenge:

48

Group:

test chemical

Dose level:

0.01 – 1%

No. with + reactions:

0

Total no. in group:

10

Clinical observations:

No erythema or edema observed

Remarks on result:

no indication of skin sensitisation

Key result

Reading:

1st reading

Hours after challenge:

48

Group:

other: control group

Dose level:

0.01-1%

No. with + reactions:

0

Total no. in group:

10

Clinical observations:

No skin reactins were observed.

Remarks on result:

no indication of skin sensitisation

Contact hypersensitivity of the test chemical

Challenge Substance	Concentration (%)	Sensitized Group		Control Group
128 -80 -3 (purified)		FR	MR	FR	MR
	1	0/10	0	0/10	0
	0.1	0/10	0	0/10	0
	0.01	0/10	0	0/10	0

Induction: 1% test chemical (commercial grade). Solvent: acetone, FR: fractional response, MR: mean response. The fractional response (FR) and mean response (MR) was observed to be 0.0 at each tested concentration.

Interpretation of results:

other: not sensitising

Conclusions:

The fractional response (FR) and mean response (MR) was observed to be 0.0 at each tested concentration.
Since no response was detected to purified test preparation (1, 0.1 and 0.01%) in test and control group , the chemical was considered to be not sensitizing to the skin of Hartley strain female albino guinea pigs.

Executive summary:

A modified guinea pig testing technique, the adjuvant and patch test was conducted on Hartley strain female albino guinea pigs to evaluate the contact hypersensitivity of test chemical.

In intradermal induction,aliquots of 0.1 ml each of a water-in-oil type emulsion (distilled water: FCA =1:1) were injected intradermally into the four corners of a previously shaved shoulder region (2 cm×4 cm). At the injection sites, scratches in the shape of agrid were made with the needle used for injection.

 

During topical induction, a closed patch with 0.1 ml of purified test preparation (1, 0.1 and 0.01%) was applied to the sites for 24 h. Abrasions and sample applications were repeated on the following 2 days. One week after the initial sensitization, 10% sodium lauryl sulfate in petrolatum was applied to the intradermal injection sites. On the next day, a closed patch of the test preparation was applied at the same sites for 48 h. After a rest period of 2 weeks, animals were challenged with the same purified test preparations (1, 0.1 and 0.01%) onto the shaved skin of the back. The excess substance was removed from the skin by washing with acetone at 24 h after the challenge application procedure.

The skin reactions, fractional response (FR) and mean response (MR), were scored at 1, 24 and 48 h after the washing.

 

The fractional response (FR) and mean response (MR) was observed to be 0.0 at each tested concentration.

Since no response was detected to purified test preparation (1, 0.1 and 0.01%) in test and control group , the chemical was considered to be not sensitizing to the skin of Hartley strain female albino guinea pigs.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not sensitising)

Additional information:

Various studies have been reviewed to evaluate the dermal sensitization potential of the test chemical in living organisms. These include in vivo experimental studies performed on guinea pigs, mice for the test chemical. The results are summarized below:

The purpose of the Local Lymph Node Assay was to identify the contact allergenic potential of the test chemical when administered to the dorsum of both ear lobes of mice. The study was performed according to OECD 429 and Commision Directive 2004/73/EC, B.42: Skin Sensitization: Local Lymph Node Assay, 29 April 2001 Guidelines.

In order to study a possible contact allergenic potential of the test chemical, three groups each of four female CBA/CaOlaHsd mice were treated daily with the test item at concentrations of 2.5 %, 5% and 10% (w/v) in acetone/olive oil (4/1, v/v) by topical application to the dorsum of each ear lobe (left and right) for three consecutive days. 10 % was the highest technically applicable concentration in the vehicle. A control group of four mice was treated with the vehicle (acetone/olive oil (4/1, v/v)) only. Five days after the first topical application the mice were injected intravenously into a tail vein with radio-labelled thymidine (3H-methyl thymidine). Approximately five hours after intravenous injection, the mice were sacrificed, the draining auricular lymph nodes excised and pooled per group. Single cell suspensions of lymph node cells were prepared from pooled lymph nodes which were subsequently washed and incubated with trichloroacetic acid overnight. The proliferative capacity of pooled lymph node cells was determined by the incorporation of 3H-methyl thymidine measured in a ß-scintillation counter. All treated animals survived the scheduled study period. No clinical signs were observed in any animals of the control group. After the first application, both ears of all test item groups of mice (Groups 2-4) showed black at dosing sites, persisting for the remainder of the in-life phase of the study. Calculation of the EC 3 value was not done because no test concentrations produced a STIMULATION INDEX (S.I.) of 3 or higher. In this study STIMULATION INDICES of 1.8, 0.8 and 1.2 were determined with the test item at concentrations of 2.5 %, 5 % and 10 % (w/v) , respectively, in acetone/olive oil (4/1 (v/v). The test chemical was therefore found to be a non-sensitizer when tested at up to the highest applicable concentration of 10 % (w/v) in acetone/olive oil (4/1 (v/v).

This is supported by a modified guinea pig testing technique, the adjuvant and patch test employed to evaluate the contact hypersensitivity of test chemical on Hartley strain female albino guinea pigs.

In intradermal induction, aliquots of 0.1 ml each of a water-in-oil type emulsion (distilled water: FCA =1:1) were injected intradermally into the four corners of a previously shaved shoulder region (2 cm×4 cm). At the injection sites, scratches in the shape of agrid were made with the needle used for injection.

During topical induction, a closed patch with 0.1 ml of purified test preparation (1, 0.1 and 0.01%) was applied to the sites for 24 h. Abrasions and sample applications were repeated on the following 2 days. One week after the initial sensitization, 10% sodium lauryl sulfate in petrolatum was applied to the intradermal injection sites. On the next day, a closed patch of the test preparation was applied at the same sites for 48 h. After a rest period of 2 weeks, animals were challenged with the same purified test preparations (1, 0.1 and 0.01%) onto the shaved skin of the back. The excess substance was removed from the skin by washing with acetone at 24 h after the challenge application procedure.

The skin reactions, fractional response (FR) and mean response (MR), were scored at 1, 24 and 48 h after the washing.

The fractional response (FR) and mean response (MR) was observed to be 0.0 at each tested concentration.

Since no response was detected to purified test preparation (1, 0.1 and 0.01%) in test and control group, the chemical was considered to be not sensitizing to the skin of Hartley strain female albino guinea pigs.

The results of the Modified Guinea pig Maximization test are further supported by a patch test performed on humans to determine the sensitization potential of the test chemical.

The dye [1% in petrolatum] was applied in Finn Chambers and read first at 2 or (more commonly) 3 days and again at 4–7 days.

The reactions of the patients were graded as ‘?+ ‘ , ‘+’ and ‘++’ categories

14 patients were tested with the dye. No patients showed any signs of sensitization. The test chemical can be considered to be non sensitizer to human skin.

Based on the available results, the test chemical can be considered to lack the potential to cause dermal sensitization. Hence, the test chemical can be considered to be not sensitizing to skin.

Respiratory sensitisation

Endpoint conclusion

Endpoint conclusion:

no study available

Justification for classification or non-classification

Based on the available results, the test chemical can be considered to lack the potential to cause dermal sensitization. Hence, the test chemical can be considered to be not sensitizing to skin.