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Diss Factsheets
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EC number: 222-294-1 | CAS number: 3407-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://www.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Experimental data of test chemical
- Justification for type of information:
- Experimental data of test chemical from collection of data
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- WoE was prepared for the determination of effect of test chemical on fish.
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- other: 1: Danio rerio (previous name: Brachydanio rerio), 2: Pimephales promelas
- Details on test organisms:
- 1: Details on test organisms
TEST ORGANISM
- Common name: zebra fish
2: Details on test organisms
TEST ORGANISM
- Common name: Fathead Minnow
- Age at study initiation (mean and range, SD): 30 days old
- Length at study initiation (length definition, mean, range and SD): 17.6 mm
- Weight at study initiation (mean and range, SD): 0.079 g - Test type:
- other: 1: static, 2: flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Post exposure observation period:
- 2: Every 24 hrs.
- Hardness:
- 2: 44.5 mg CaCO3/l
- Test temperature:
- 2: 24.4 ° C
- pH:
- 2: 7.7
- Dissolved oxygen:
- 2: 6.8 mg/L
- Nominal and measured concentrations:
- 2: 0, nominal 5 concentrations in the range of 4.39 to 24.6 mg/l tested
- Details on test conditions:
- 2: Details on test conditions:
Alkalinity: 44.5 mg CaCO3/l - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 1st study
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 2nd study
- Details on results:
- 1: Slow and inactive swimming behaviour and loss of equilibrium (uncontrolled movements) were also observed.
2: Details on result
- Behavioural abnormalities: Affected fish lost schooling behavior, were hypoactive and underreactive to external stimuli. They had increased respiration were darkly colored and lost equilibrium prior to death. - Reported statistics and error estimates:
- 1: Probit analysis
- Sublethal observations / clinical signs:
1:
Fig: Effect concentration on the growth of fish.
Hours
Mortality rate of fish
0
0 %
24
80 %
48
90 %
72
90 %
96
90 %
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 1: Based on the mortality of freshwater fish Brachydanio rerio by the chemical exposure for 96 hrs, the LC50 was determine to be 17.6 mg/l and the LC0 and LC100 was 11.3 mg/l and 26.2 mg/l.
2: Based on the mortality of fishes Pimephales promelas after the exposure of chemical for 96 hrs, the LC50 was determine to be 18.9 mg/l.
Thus based on the above both studies, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria. - Executive summary:
Data available for the test chemicals has been reviewed to determine its effect on the mortality of fishes. The studies are as mentioned below:
Aim of this study was to determine the nature of chemical on the mortality of freshwater fish Brachydanio rerio for total exposure of 96 hrs. Chemical was analytically monetarized by GC. Test conducted under static system. After incubation period of 96 hrs, slow and inactive swimming behaviour and loss of equilibrium (uncontrolled movements) were also observed. Based on the mortality of freshwater fish Brachydanio rerio by the chemical exposure for 96 hrs, the LC50 was determine to be 17.6 mg/l and the LC0 and LC100 was 11.3 mg/l and 26.2 mg/l. Based on the LC50, chemical was consider as toxic and classified in aquatic chronic 3 category as per the CLP classification criteria.
Similar study was also conducted on other fish. Aim of this study was to determine the nature of chemical on the mortality of fish Pimephales promelas for total exposure of 96 hrs. Study was conducted comparable to standard method and in accordance with general accepted scientific standards. Nominal 5 concentrations in the range of 4.39 to 24.6 mg/l were used and the sample was analytically monetarized by GC method. 0.079 g 30 days old Pimephales promelas (Fathead Minnow) were used. Test conducted under the flow- through system. Observations of fish behaviour and body morphology at regular intervals. Affected fish lost schooling behavior, were hypoactive and underreactive to external stimuli. They had increased respiration were darkly colored and lost equilibrium prior to death. Based on the mortality of fishes Pimephales promelas after the exposure of chemical for 96 hrs, the LC50 was determine to be 18.9 mg/l. based on the LC50, chemical was consider as toxic and classified as aquatic chronic 3 category as per the CLP classification criteria.
Thus based on the above both studies, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.
Reference
Description of key information
1: Based on the mortality of freshwater fish Brachydanio rerio by the chemical exposure for 96 hrs, the LC50 was determine to be 17.6 mg/l and the LC0 and LC100 was 11.3 mg/l and 26.2 mg/l.
2: Based on the mortality of fishes Pimephales promelas after the exposure of chemical for 96 hrs, the LC50 was determine to be 18.9 mg/l.
Thus based on the above both studies, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 17.6 mg/L
Additional information
Data available for the test chemical, structually and functionally similar read across chemicals has been reviewed to determine its effect on the mortality of fishes. The studies are as mentioned below:
Aim of this study was to determine the nature of chemical on the mortality of freshwater fish Brachydanio rerio for total exposure of 96 hrs. Chemical was analytically monetarized by GC. Test conducted under static system. After incubation period of 96 hrs, slow and inactive swimming behaviour and loss of equilibrium (uncontrolled movements) were also observed. Based on the mortality of freshwater fish Brachydanio rerio by the chemical exposure for 96 hrs, the LC50 was determine to be 17.6 mg/l and the LC0 and LC100 was 11.3 mg/l and 26.2 mg/l. Based on the LC50, chemical was consider as toxic and can be consider to to be classfied as aquatic chronic 3 category as per the CLP classification criteria.
Similar study was also conducted on other fish. Study was to determine the nature of chemical on the mortality of fish Pimephales promelas for total exposure of 96 hrs. Study was conducted comparable to standard method and in accordance with general accepted scientific standards. Nominal 5 concentrations in the range of 4.39 to 24.6 mg/l were used and the sample was analytically monetarized by GC method. 0.079 g 30 days old Pimephales promelas (Fathead Minnow) were used. Test conducted under the flow- through system. Observations of fish behaviour and body morphology at regular intervals. Affected fish lost schooling behavior, were hypoactive and underreactive to external stimuli. They had increased respiration were darkly colored and lost equilibrium prior to death. Based on the mortality of fishes Pimephales promelas after the exposure of chemical for 96 hrs, the LC50 was determine to be 18.9 mg/l. based on the LC50, chemical was consider as toxic and can be consider to not classified as aquatic chronic 3 category as per the CLP classification criteria.
Thus based on the above both studies, chemical consider to be toxic and classified as aquatic chronic 3 as per the CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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