Registration Dossier

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Biodegradation in water:

28-days Closed Bottle test following the OECD guideline 301 D was performed to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used as inoculum with bacterial count as 107 to 108 CFU/ml.The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32ml/L . OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 73.49 %. Degradation of Sodium Benzoate exceeds 39.15 % on 7 days & 61.44 on 14th day. The activity of the inoculum is thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.42 mgO2/mg. ThOD was calculated as 3.04 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 13.81 %. Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable in nature.

Biodegradation in water and sediments:

Estimation Programs Interface (EPI Suite, 2018) prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 17.1 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 37.5  days (900 hrs). The half-life (37.5  days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is low , whereas the half-life period of test chemical in sediment is estimated to be  337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 3 % (i.e, reported as  2.71 %), indicates that test chemical is not persistent in sediment.

Biodegradation in soil:

The half-life period of test chemicaln soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2018). If released into the environment, 79.9 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is low .

Additional information

Biodegradation in water:

Experimental studies for test chemical have been performed for biodegradadtion endpoint and their results are summarized below.

In first study the 28-days Closed Bottle test following the OECD guideline 301 D was performed to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used as inoculum with bacterial count as 107 to 108 CFU/ml. .The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32ml/L . OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 73.49 %. Degradation of Sodium Benzoate exceeds 39.15 % on 7 days & 61.44 on 14th day. The activity of the inoculum is thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.42 mgO2/mg. ThOD was calculated as 3.04 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 13.81 %. Based on the results, the test item, under the test conditions, was considered to be not readily biodegradable in nature.

Another study wa sre viewed from authoritative database in this study the Biodegradation experiment was carried out for determining biodegradability of test chemical in this experiment non adapted activated sludge was used as inoculums at 30 mg/L concentration for 28 days. Biodegradation was analyzed by using two parameters namely O2 cosumption and test material analysis by GC. The initial concentration of test chemical was 100 mg/L. After 28 days of incubation Percent biodegradation of test chemical was observed to be 0.0% by by O2 consumption parameter and 3% test material analysis by GC parameter in 28 days. Therefore it is concluded that test chemical is not readily biodegradable.

On the basis of results of both the studies mentioned above it is concluded that test chemical is not readily biodegradable.

Biodegradation in water and sediments:

Estimation Programs Interface (EPI Suite, 2018) prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 17.1 % of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 37.5  days (900 hrs). The half-life (37.5  days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is low , whereas the half-life period of test chemical in sediment is estimated to be  337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 3 % (i.e, reported as  2.71 %), indicates that test chemical is not persistent in sediment.

Biodegradation in soil:

The half-life period of test chemicaln soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2018). If released into the environment, 79.9 % of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of test chemical in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of test chemical, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is low .