Methylpentynol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (only 4 strains tested, without E. Coli)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

only 4 strains tested, without E.Coli

Principles of method if other than guideline:

BASF test

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Methylpentynol

EC Number:

201-055-5

EC Name:

Methylpentynol

Cas Number:

77-75-8

Molecular formula:

C6H10O

IUPAC Name:

3-methylpent-1-yn-3-ol

Details on test material:

purity 99.9%

Method

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium TA1535, TA100, TA1537, TA98

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

with and without

Metabolic activation system:

S9-Mix

Test concentrations with justification for top dose:

20-5000 µg/plate

Vehicle / solvent:

dest. water

Controls

Controls 1

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

with metabolic activation

Controls 2

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: N-methyl-N-nitro-N-nitroso-guanidine for the strains TA 100 and TA 1535

Remarks:

without metabolic activation

Controls 3

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylendiamine for the strain TA 98

Remarks:

without metabolic activation

Controls 4

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

Remarks:

without metabolic activation

Migrated to IUCLID6: for the strain TA 1537

Details on test system and experimental conditions:

Ames test

Evaluation criteria:

In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:

-doubling of the spontaneous mutation rate (control)
-dose-response relationship
-reproducibility of the results

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity nor precipitates, but tested up to recommended limit concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: other: Salmonella typhimurium TA1535,TA100,TA1537, TA98

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

negative

Executive summary:

Methylpentinol was tested for mutagenicity in the Ames test. An increase in the number of his+ revertants was not observed both in the standard plate test and in the preincubation test either without S-9 mix or after the addition of a metabolizing system. According to the results of the present study, the test substance Methylpentinol is not mutagenic in the Ames test under the experimental conditions chosen here.