3-chloropropyne

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP non-guideline, available as unpublished report, limitations in design and/or reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1955

Report date:

1955

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Principles of method if other than guideline:

Method: Before OECD Guideline 404 was established in 1982, skin irritation was tested using an internal method (BASF test). White Vienna rabbits were used. Usually, 2 animals were treated for 1, 5 and 15 minutes and 2 other animals for 20 hours using occlusive conditions. An application site of 2.5 X 2.5 cm was covered with the liquid or powdered and moistened test substance. After the application time, the skin was washed with water which sometimes contained a mild detergent (e.g. Lutrol (50%)). The animals were observed 7 to 8 days and skin changes were recorded on working days. The report usually describes findings after 24 hours and at the end of the observation period.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropyne

EC Number:

210-856-9

EC Name:

3-chloropropyne

Cas Number:

624-65-7

Molecular formula:

C3H3Cl

IUPAC Name:

3-chloroprop-1-yne

Details on test material:

- Name of test material (as cited in study report): Propargylchlorid

Test animals

Species:

rabbit

Strain:

Vienna White

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

other: alcohol

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Experiment 1, concentration: 0.1, 0.5, 1, 10, 20, 50, 100 %
- Experiment 2, concentration: 100 %

VEHICLE
- Experiment 1, concentration: 90%

Duration of treatment / exposure:

- Experiment 1 (0.1, 0.5, 1, 10, 20, 50, 100 % test material): 20 hours
- Experiment 2 (100% test material): 1, 5, 15, or 60 minutes

Observation period:

6 weeks

Number of animals:

- Experiment 1: 2 (0.1, 0.5, 1, 10, 100 %) or 4 (20, 50 %)
- Experiment 2: 2 per time point

Details on study design:

TEST SITE
- Area of exposure: 2.5 x 2.5 cm (patch test)
- Type of wrap if used: bandage

REMOVAL OF TEST SUBSTANCE
- After the application time, the skin was washed

Results and discussion

In vivo

Irritant / corrosive response data:

More than 1 animal had necrosis:
- Experiment 1: necrosis observed in 2 out of 4 animals, in the 20 and 50 % group
- Experiment 2: necrosis observed in 2 out of 2 animals, in the 60 min group

Edema was observed in all animals exposed to 50 % and 100% of the test material.

Any other information on results incl. tables

Results after 20 hours exposure (Experiment 1)

Concentration (100%)	Effects
100	Redness, oedema, crusts, scaling
50	Redness, oedema, crusts, scaling, necrosis, scarring
20	Redness, crusts, scaling, necrosis, scarring
10	Slight redness, scaling
1	Slight redness
0.5	No irritation
0.1	Slight redness

Results after exposure with pure test material (Experiment 2)

Exposure duration
1 min	5 min	15 min	60 min
Redness, oedema, crusts, scaling, necrosis, subcutaneous haemorrhage	Redness, oedema, crusts, scaling, necrosis, scarring, subcutaneous haemorrhage	Redness, oedema, crusts, scaling, necrosis, scarring, subcutaneous haemorrhage	Redness, oedema, crusts, necrosis, subcutaneous haemorrhage

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information