3-chloropropyne

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study predating currently accepted guidelines and with unknown GLP status. Limitations in design and reporting but results considered reliable and adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Test principle:
In the respirometry test the biodegradation of test substance and the toxicity of test substance towards activated sludge microorganisms is examined. In closed flasks, mixtures of activated sludge, nutrient salts, the test substance and a reference substance are incubated for 28 days under controlled conditions. The biochemical oxygen demand (BOD) is measured throughout the test and at the end of the incubation period the dissolved organic carbon content in the test flaks is determined.
A toxicological effect is defined as inhibition of respiration of the activated sludge which is measured as BOD values at t=24h and to account for possible adaptation effects, also at the end of the test. The respiration inhibition in the flasks containing activated sludge, test substance and reference substance is compared to flasks containing activated sludge and reference substance only.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-chloropropyne

EC Number:

210-856-9

EC Name:

3-chloropropyne

Cas Number:

624-65-7

Molecular formula:

C3H3Cl

IUPAC Name:

3-chloroprop-1-yne

Details on test material:

- Name of test material (as cited in study report): Propargylchlorid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly industrial sewage

Details on inoculum:

- Adaptation: no detail
- Pre-treatment: 2 washings
- Dryweight: 4000 mg/L

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

23 d

Test conditions

Nominal and measured concentrations:

Test substance: TOC of 29, 49, 99, 496 and 992 mg/L (prepared with 0.06, 0.10, 0.20, 1.00 and 2.00 mL/L test substace)
Reference substance: TOC of 120 mg/L

Reference substance (positive control):

yes

Remarks:

unspecified "gut abbaubarem testsubstrat".

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

other: EC20

Effect conc.:

0.26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

7 d

Dose descriptor:

other: EC20

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 4

Duration:

7 d

Dose descriptor:

EC50

Effect conc.:

1.22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 5

Duration:

28 d

Dose descriptor:

other: EC20

Effect conc.:

0.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate