Disulphur dichloride

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

short-term repeated dose toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

This study is not reliable due to methodological deficiencies and therefore inappropriate for a toxicological assessment of disulphur dichloride. (No analytical verification of test atmosphere, solvent methylene chloride; one rat, one rabbit and one guinea pig histopathologically investigated and autopsy reported to showed pulmonary and bronchial inflammation, congestion and strong inflammatory symptoms in the kidneys and effects on liver and kifney (no further data). in rat and rabbits. No effects were reported in guinea pig; Gross 1944.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1944

Report date:

1944

Materials and methods

Principles of method if other than guideline:

2 rabbits, 2 guinea pigs, 2 rats were exposed in a 2 m³ vessel to disulphur dichloride vapour of 0.1 mm³/L for 4 hours. The test was repeated per over 25 days. Observation for clinical signs, post exposure histopathological examination.

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study

Test material

Test material information

Constituent 1

Reference substance name:

Disulphur dichloride

EC Number:

233-036-2

EC Name:

Disulphur dichloride

Cas Number:

10025-67-9

Molecular formula:

Cl2S2

IUPAC Name:

dichlorodisulfane

Details on test material:

- Name of test material (as cited in study report): disulphur dichloride
- Analytical purity: not reported

Test animals

Species:

other: rat, rabbit, guinea pig

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

other: 10 % solution of test substance in dichloromethane

Remarks on MMAD:

MMAD / GSD: not applicable

Details on inhalation exposure:

Daily exposure was 4 hour, after every hour the test chamber was ventilated and the test atmosphere reconstituted with 0.1 mm³/liter test substance

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

25 days

Frequency of treatment:

4 hours/day over 25 day-exposure

Doses / concentrations

Remarks:

Doses / Concentrations:
0.1 mm³/L dissolved in methylene chloride
Basis:
nominal conc.

Control animals:

other: no data specified

Details on study design:

Post-exposure period: no

Positive control:

no data

Examinations

Observations and examinations performed and frequency:

CAGE SIDE OBSERVATIONS: Yes

Sacrifice and pathology:

All animals except 1 rabbit which died after 14 days received 25 exposures. Afterwards the second rabbit, 1 guinea pig and 1 rat were sacrified and examined histopatologically.

Other examinations:

no data

Statistics:

no data

Results and discussion

Results of examinations

Details on results:

The most of the animals showed no symptoms during the duration of the experiments. However, in 1 rabbit increased protein content in urine on day 12 of exposure, it visibly lost weight and was found dead on the morning after the 14th day of the experiment. The autopsy showed pulmonary and bronchial inflammation, congestion and strong inflammatory symptoms in the kidneys (glomorulo-tubular nephritis with bleeding). After cessation of the exposure 1 rabbit, 1 guinea pig and 1 rat were examined histopathologically. Effects on liver and kidney (no further data) in rat and rabbits were noted. No effects were reported in guinea pig.

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

The most of the animals showed no symptoms during the duration of the experiments. However, in 1 rabbit increased protein content in urine on day 12 of exposure, it visibly lost weight and was found dead on the morning after the 14th day of the experiment. The autopsy showed pulmonary and bronchial inflammation, congestion and strong inflammatory symptoms in the kidneys (glomorulo-tubular nephritis with bleeding). After cessation of the exposure 1 rabbit, 1 guinea pig and 1 rat were examined histopathologically. Effects on liver and kidney (no further data) in rat and rabbits were noted. No effects were reported in guinea pig.

Applicant's summary and conclusion

Executive summary:

2 rabbits, 2 guinea pigs, 2 rats were exposed in a 2 m³ vessel to disulphur dichloride vapour of 0.1 mm³/L for 4 hours. The test was repeated per over 25 days. Observation for clinical signs, post exposure histopathological examination.

The most of the animals showed no symptoms during the duration of the experiments. However, in 1 rabbit increased protein content in urine on day 12 of exposure, it visibly lost weight and was found dead on the morning after the 14th day of the experiment. The autopsy showed pulmonary and bronchial inflammation, congestion and strong inflammatory symptoms in the kidneys (glomorulo-tubular nephritis with bleeding). After cessation of the exposure 1 rabbit, 1 guinea pig and 1 rat were examined histopathologically. Effects on liver and kidney (no further data) in rat and rabbits were noted. No effects were reported in guinea pig.

This study is not reliable due to methodological deficiencies and therefore inappropriate for a toxicological assessment of disulphur dichloride. (No analytical verification of test atmosphere, solvent methylene chloride; one rat, one rabbit and one guinea pig histopathologically investigated and autopsy reported to showed pulmonary and bronchial inflammation, congestion and strong inflammatory symptoms in the kidneys and effects on liver and kifney (no further data). in rat and rabbits. No effects were reported in guinea pig; Gross 1944.