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EC number: 255-460-7 | CAS number: 41611-76-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Macrolex Blau 3R was initially investigated using the Salmonella/microsome plate incorporation test for point mutagenic effects, dissolved in DMSO, in doses of up to and including 5000 µg per plate on five Salmonella typhimurium LT2 mutants. These comprised the histidine-auxotrophic strains TA 1535, TA 100, TA 1537, TA 98 and TA 102. The independent repeat experiment was performed as preincubation modification for 20 minutes at 37 °C in the absence of S9 mix in doses of up to and including 1600 µg per plate and in the presence of S9 mix of up to and including 5000 µg per plate.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,4-bis[(2-ethyl-6-methylphenyl)amino]anthraquinone
- EC Number:
- 255-460-7
- EC Name:
- 1,4-bis[(2-ethyl-6-methylphenyl)amino]anthraquinone
- Cas Number:
- 41611-76-1
- Molecular formula:
- C32H30N2O2
- IUPAC Name:
- 1,4-bis[(2-ethyl-6-methylphenyl)amino]-9,10-dihydroanthracene-9,10-dione
- Details on test material:
- Name of test substance : Macrolex Blau 3R
Further name of test substance: Reinblau RLW
Batch number : CHN 23196
Content : 99.5 %
Visual appearance : blue powder
Chemical name : 1,4-Bis[(2-ethyl-6-methylphenyl)amino]-9,10-anthracenedione
Molecular weight : 474.6 g/mol
Molecular formula : C32H30N2O2
CAS-No. : 41611-76-1
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Additional strain / cell type characteristics:
- other: Firstly, they are deep rough since certain lipopolysaccharide side chains are missing in the bacterial cell wall. Secondly, their reduced ability to repair damage from UV light (e.g. thymidine dimers) allows the phenotypic detection of mutation events
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix
- Test concentrations with justification for top dose:
- Plate incorporation method: with and without S9 mix: 0, 50, 160, 500, 1600, 5000 µg/plate
Preincubation method: with S9 mix: 0, 50, 160, 500, 1600, 5000 µg/tube; without S9 mix 0, 16, 50, 160, 500, 1600 µg/tube
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: sodium azide, 4-nitro-1,2-phenylene diamine, 2-nitrofluoren, mitomycon c, cumene hydroperoxide, 2-aminoanthracene
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- Doses up to and including 5000 µg per plate did not cause any bacteriotoxic effects
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The Salmonella/microsome plate incorporation test, employing doses of up to 5000 µg per plate, showed Macrolex Blau 3R not to produce bacteriotoxic effects. Substance precipitation occurred without S9 mix at 500 µg per plate and above and with S9 mix only at 5000 µg per plate.
Evaluation of individual dose groups, with respect to relevant assessment parame-ters (dose effect, reproducibility) revealed no biologically relevant variations from the respective negative controls.
In spite of the low doses used, positive controls increased the mutant counts to well over those of the negative controls, and thus demonstrated the system's high sensitivity.
Despite this sensitivity, no indications of mutagenic effects of Macrolex Blau 3R could be found at assessable doses of up to 5000 µg per plate in any of the Salmonella typhimurium strains.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative - Executive summary:
Macrolex Blau 3R was initially investigated using the Salmonella/microsome plate incorporation test for point mutagenic effects, dissolved in DMSO, in doses of up to and including 5000 µg per plate on five Salmonella typhimurium LT2 mutants. These comprised the histidine-auxotrophic strains TA 1535, TA 100, TA 1537, TA 98 and TA 102. The independent repeat experiment was performed as preincubation modification for 20 minutes at 37 °C in the absence of S9 mix in doses of up to and including 1600 µg per plate and in the presence of S9 mix of up to and including 5000 µg per plate. Other conditions remained unchanged.
Doses up to and including 5000 µg per plate did not cause any bacteriotoxic effects. Total bacteria counts remained unchanged and no inhibition of growth was observed. Substance precipitation occurred without S9 mix at the dose of 500 µg per plate and above and with S9 mix at 5000 µg per plate.
Evidence of mutagenic activity of Macrolex Blau 3R was not seen. No biologically relevant increase in the mutant count, in comparison to the negative controls, was observed in any of the strains tested, without and with S9 mix, in the plate incorporation as well as in the preincubation modification, under the experimental conditions applied.
The positive controls sodium azide, 4-nitro-1,2-phenylene diamine, 2-nitrofluorene, mitomycin C, cumene hydroperoxide and 2-aminoanthracene had a marked mutagenic effect, as was seen by a biologically relevant increase in mutant colonies compared to the corresponding negative controls.
Therefore, Macrolex Blau 3R is negative in the Salmonella/microsome test.
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