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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, GLP, All validity criteria fulfilled, complete identification of test substance, including chemical analyses
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
The short term toxicity test result as observed for the tallow based product is read across to the Oleyl based product. The products differ only in their alkylchain distribution. Both tallow and Oleyl are natural fats for which the composition slightly changes over time due to changes in the environmental considitions.

The tallow alkyl fat which is used for the production of CAS no 1268344-02-0 are for 99 % straight chain alkyl fats

Commercial tallow alkyl fats have the following distribution:

C12 about 2 %
C14 about 4 %
C16 about 31 %
C18 about 65 %,
where 40 –50 % are unsaturated.

The oleyl fate which is used for the production of CAS no 1290049-56-7 are also straight chain fates

Commercial Oleylfats have the following distribution:

C12 1 %
C14 2 - 4 %
C16 and C16’ 12 - 14 %
C18, C18’ and C18’’ > 80 % (C18: 8 %, C18’cis: 46 %, C18’trans: 21 %, C18’’: 5 %)
C20 1 %
(a single prime indicates one double bond, a double prime indicates two double bonds):

When the water solubility of cationic surfactants based on tallow are compared to oleyl based cationic surfactants it is consistently the tallow product which has a sligthly lower water solubility and due to that also a slightly lower bioavailability. This lower bioavailability regularly results is slight differences in biodegradation rate and ecotoxicity. The substances are however difficult to test substances and the variation in the test results is especially in ecotoxicity testing frequently larger than the difference in bioavailability. It is therefore considered justified to use the tallow product test results for read across for the oleyl based product.
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: OECD TG 211
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Test organisms (species):
Daphnia magna
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Reference substance (positive control):
no
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
421 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Description of key information

Short term (48-h) toxicity to Daphnia magna was investigated in accordance with the guideline OECD 202 with a tallow based ethoxylated diamine but without  confirmation of the exposure concentrations by chemical analysis. To support the use of this tallow based result for read-across to the oleyl based ethoxylated diamine without additional safety factor, the 21d-EC50 for parental mortality of 421 µg/L as observed for the oleyl based ethoxylated diamine is included to the information used to evaluate the acute effects on daphnia. The lowest value will as a worst-case be used for risk assessment.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.31 mg/L

Additional information

The short-term tests on toxicity to Daphnia magna were conducted in a period when no reliable specific method of analyses was available. The concentrations were therefore not analytically verified, the reliability of the results is therefore limited.