1,1-Bis(acryloyloxymethyl)ethyl isocyanate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 Jun - 07 Jul 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Staatliches Gewerbeaufsichtsamt Hildesheim

Test material

Test material information

Constituent 1

Reference substance name:

2-isocyanato-2-methyl-3-(prop-2-enoyloxy)propyl prop-2-enoate

Cas Number:

886577-76-0

Molecular formula:

C11H13NO5

IUPAC Name:

2-isocyanato-2-methyl-3-(prop-2-enoyloxy)propyl prop-2-enoate

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Municipal sewage treatment plant, 31137 Hildesheim, Germany- Initial cell/biomass concentration: Approx. 1.30 x 10E+07 cfu/L in the final test solution- Pretreatment: Activated sludge was washed twice with chlorine free tap water; settled sludge resuspended in mineral salts medium; maintained under aerobic condition by aeration for 2 h; homogenized with a blender; after sedimentation the supernatant decanted and maintained under aerobic conditions by aeration with CO2-free air for 6 days; 10 mL/L used to initiate inoculation

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

36 mg/L

Based on:

test mat.

Remarks:

corresponding to an oxygen demand of 52.2 mg O2/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS- Composition of medium: Mineral salts medium according to OECD 301 F- Test temperature: 20 - 24 °C- pH: 7.57 - 7.83- pH adjusted: no- Dispersion treatment: Continuous stirring- Continuous darkness: yesTEST SYSTEM- Measuring equipment: OxiTop system- Test performed in closed vessels: yes- Details of trap for CO2 and volatile organics if used: Soda limeSAMPLING- Sampling frequency: Continuous measurement (360 measuring points during the 28 days; every 112 min one measurement)CONTROL AND BLANK SYSTEM- Inoculum control: yes (with 2 replicates)- Toxicity control: yes- Functional control: yes

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

32

Sampling time:

28 d

Details on results:

In the toxicity control (36 mg/L test item and 45 mg/L sodium benzoate) the biodegradation achieved 59% after 14 days. After 28 days the biodegradation came to 69%. The validity criterion that the test item should not inhibit the biodegradation of the reference item was fulfilled.

BOD5 / COD results

Results with reference substance:

The adaptation phase changed to degradation phase within 1 day (degradation > 10 %) and the biodegradation came to a maximum of 100 % on day 27. The pass level for ready biodegradation (> 60% degradation) was reached on day 2. The validity criterion that the degradation should be> 60% after 14 d was fulfilled.

Any other information on results incl. tables

Table 1: Degradation of test substance, functional- and toxicity control

Biodegradation (%)
	Day 7	Day 14	Day 21	Day 28
Replicate 1 Test Item 36 mg/L Replicate 2	7	15	21	26
	9	25	31	37
Functional Control 45 mg/L	87	94	97	100
Toxicity Control 36 mg/L Test Item + 45 mg/L Reference Item	46	59	66	69

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

not readily biodegradable: 32% after 28 d (OECD 301F)