Strontium 4-[(4-chloro-5-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate (1:1)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Barium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate

EC Number:

231-494-8

EC Name:

Barium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate

Cas Number:

7585-41-3

Molecular formula:

C18H13ClN2O6S.Ba

IUPAC Name:

barium 4-[(5-chloro-4-methyl-2-sulfonatophenyl)diazenyl]-3-hydroxy-2-naphthoate

Test material form:

solid: nanoform

Details on test material:

Test materials used in this dossier are all considered to fall under the definition of nano-materials according to the European Commission Recommendation 2011/696/EU as the synthesis and manufacturing of this pigment always yields particulate material with a fine particle size distribution.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1.00 g test substance were added to 10 L test water and the mixture was homogenized with an ultra-turrax stirrer. Since the test substance was sparingly soluble in water, the test concentration was prepared 1 day before the test organisms were introduced into the test vessel (study start) and stirred during this time, to ensure that saturated concentration was used.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: breed in testing facility
- Age at study initiation: about 7 months
- Length at study initiation: 3.6 (3.4 - 3.7) cm
- Weight at study initiation: 0.54 (0.43 - 0.63) g
- Food type: Standard feed (TETRA MIN), on workdays generally additionally live artemia
- Amount: ad libitum

ACCLIMATION
- Conditions equivalent to housing
- Health during acclimation (any mortality observed): 0 % mortality during the last 7 days prior to test initiation

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

- Total hardness: about 2.5 mM (about 250 mg/L CaCO3)
- Acid capacity: about 5.5 mM

Test temperature:

22 - 23 °C

pH:

8.1 - 8.2

Dissolved oxygen:

8.4 - 8.5 mg/L

Nominal and measured concentrations:

The following nominal concentration were tested: 0, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel type: open
- Material, size, headspace, fill volume: all-glass aquaria, 30 x 22 x 24 cm, 10 L fill volume (water depth about 17 cm)
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Biomass loading rate: ca. 0.5 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated, char coal filtered
- Ca/Mg ratio: 9:1
- Intervals of water quality measurement: after 1, 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

EFFECT PARAMETERS MEASURED: mortality and symptoms

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: no effects observed up to the limit of water solubility.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

With high probability acutely not toxic to fish.