Sodium 3-nitrobenzenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from BASF

Qualifier:

according to guideline

Guideline:

other: Refer below

Principles of method if other than guideline:

Aim of this study was to determine the effect of test chemical on the growth of fish.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fa. P. Eggers, Hohenweststedt, bei Neumünster, FRG
- Length at study initiation: 4.4 cm
- Weight at study initiation: 0.96 g
- Corpulence factor of the batch: 1.13


ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: Holstenstolz, Alleinfutter für Forellen; Typ 34; purchased from Wilhelm Stroeh Jun. KG, FRG
- Feeding frequency: Ad libitum

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

2.6 mmol/L
5.2 mval/L

Test temperature:

20 ± 1°C

pH:

7.7 - 7.9

Dissolved oxygen:

8.3 - 8.9 mg/L

Nominal and measured concentrations:

Nominal concentrations: Control and 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L glass aquaria (30 x 22 x 24 cm)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Aeration: Continuous with oil-free air

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Alkalinity: 0.8 mmol/L
- Intervals of water quality measurement: Daily

EFFECT PARAMETERS MEASURED: Mortality, symptoms

Reference substance (positive control):

yes

Remarks:

(Chloroacetamide)

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: None
- Mortality of control: None

Validity criteria fulfilled:

not specified

Conclusions:

The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus) in a 96 hr study on mortality effect was determine to be > 500 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus) within the dose levels mentioned in the study.

Executive summary:

Aim of this study was to determine the effect of test chemical on the growth of fish.The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus) in a 96 hr study on mortality effect was determine to be > 500 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus) within the dose levels mentioned in the study.

Description of key information

Aim of this study was to determine the effect of test chemical on the growth of fish.The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus) in a 96 hr study on mortality effect was determine to be > 500 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus) within the dose levels mentioned in the study.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

500 mg/L

Additional information

Based on the various experimental data for the test chemical study have been reviewed to determine the toxic nature of test chemical on the growth of fish. The studies are as mentioned below:

 

Aim of this study was to determine the effect of test chemical on the growth of fish.The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus) in a 96 hr study on mortality effect was determine to be > 500 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus) within the dose levels mentioned in the study.

 

First experimental study was supported by the second supporting study from peer reviewed journal The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus, Brachydanio rerio, Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulata) in a 24-96 hr study on mortality effect was determine to be >500 - 1350 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus, Brachydanio rerio, Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulata). Thus based on the LC50 chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

Similarly in the third supporting study from journal short term toxicity of test chemical was studied on the growth of Lepomis macrochirus under the static system. The lethal concentration (LC50) value of test chemical in fish (Lepomis macrochirus) in a 24 hr study on mortality effect was determine to be < 1350 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Lepomis macrochirus).

 

The fourth study from other database were used for the test chemical which supports the nontoxic nature of chemical. Short term study was carried out on the growth of fish. The lethal concentration (LC50) value of test chemical in fish (Alburnus alburnus) in a 96 hr study on mortality effect was determine to be 5500 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance , it is concluded that test chemical does not exhibit short term toxicity to fish (Alburnus alburnus).

 

Based on the experimental studies from various sources, it was concluded that the test chemical is nontoxic and not classified as per the CLP classification criteria.