Sodium 3-nitrobenzenesulphonate

Endpoint summary

Description of key information

Short term toxicity to fish:

Aim of this study was to determine the effect of test chemical on the growth of fish.The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus) in a 96 hr study on mortality effect was determine to be > 500 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus) within the dose levels mentioned in the study.

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test test substance. On the basis of no effects observed in a static freshwater fish, the NOEC value for the substance is estimated to be 115000 mg/l for fish for 28 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

Short term toxicity to invertebrates:

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The solution of white pearl 100 mg/l was prepared by dissolving the substance in reconstituted water. Test conducted at the limit concentration 100 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Based on the immobility of daphnia magna due to the exposure of chemical for 48 hrs, the inhibitory concentration IC were observed at 100 mg/l. At 100 mg/l only 8 % inhibition was observed in daphnia magna thus it was concluded that the EC50 was > 100 mg/l. Based on the above effects it was concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria. 

 

Long term toxicity to aq. invertebrates:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrates was predicted for test substance. On the basis of no effects observed on a freshwater aquatic invertebrates, the NOEC value for the substance is estimated to be 29119.572 mg/l for daphnia for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to daphnia at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

 

 

Toxicity to algae:

Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The solution of white pearl 100 mg/l was prepared by dissolving the substance in OECD growth medium. Test conducted at limit concentration of 100 mg/l. Strain 86.81 SAG were used in the study. 5x10(3) cells/ml were added in 100 ml test vessel. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, inhibitory concentration IC was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. After the exposure of chemical with algae Desmodesmus subspicatus, no inhibition were observed. And the IC0% was determine at 100 mg/l. As no effect were observed at limit dose 100 mg/l thus it was concluded that the EC50 was > 100 mg/. Based on the no effect on the growth of algae, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

 

 

 

Toxicity to microorganisms:

In the first key study for the test chemical used from peer reviewed journal toxicity were measured. Short term toxicity of chemical was studied on the growth of bacteria. The effect concentration (EC50) value of test chemical on microorganism (Bacteria sp.) in a 17 hrs study was observed at a dose concentration of > 10000 mg/L.

Additional information

Summarized result for the determination of nature of chemical on the growth and other biological and physical activity of fishes, aquatic invertebrates, algae and cyanobacteria and microorganisms when chemical comes in contact with test organisms, by considering the data for test chemical as well as structurally and functionally similar read across chemicals are as follows:  

 

Short term toxicity to fish:

Based on the various experimental data for the test chemical study have been reviewed to determine the toxic nature of test chemical on the growth of fish. The studies are as mentioned below:

 

Aim of this study was to determine the effect of test chemical on the growth of fish.The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus) in a 96 hr study on mortality effect was determine to be > 500 mg/L.Thus,considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus) within the dose levels mentioned in the study.

 

First experimental study was supported by the second supporting study from peer reviewed journal The lethal concentration (LC50) value of test chemical in fish (Leuciscus idus, Brachydanio rerio, Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulata) in a 24-96 hr study on mortality effect was determine to be >500 - 1350 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Leuciscus idus, Brachydanio rerio, Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulata). Thus based on the LC50 chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

Similarly in the third supporting study from journal short term toxicity of test chemical was studied on the growth of Lepomis macrochirus under the static system. The lethal concentration (LC50) value of test chemical in fish (Lepomis macrochirus) in a 24 hr study on mortality effect was determine to be < 1350 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to fish (Lepomis macrochirus).

 

The fourth study from other database were used for the test chemical which supports the nontoxic nature of chemical. Short term study was carried out on the growth of fish. The lethal concentration (LC50) value of test chemical in fish (Alburnus alburnus) in a 96 hr study on mortality effect was determine to be 5500 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance , it is concluded that test chemical does not exhibit short term toxicity to fish (Alburnus alburnus).

 

Based on the experimental studies from various sources, it was concluded that the test chemical is nontoxic and not classified as per the CLP classification criteria.

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test test substance. On the basis of no effects observed in a static freshwater fish, the NOEC value for the substance is estimated to be 115000 mg/l for fish for 28 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

 

Short term toxicity to invertebrates:

Summarized result for the toxicity of chemical on the growth of aquatic invertebrates are as follows:

 

In the first key study from toxicity were measured. Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The solution of white pearl 100 mg/l was prepared by dissolving the substance in reconstituted water. Test conducted at the limit concentration 100 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Based on the immobility of daphnia magna due to the exposure of chemical for 48 hrs, the inhibitory concentration IC were observed at 100 mg/l. At 100 mg/l only 8 % inhibition was observed in daphnia magna thus it was concluded that the EC50 was > 100 mg/l. Based on the above effects it was concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria. 

 

Similarly in the second supporting study for the test chemical from peer reviewed journal measured the toxicity. Aim of this study was to determine the nature and behavior of chemical when comes in contact with the test organism daphnia magna. Study conducted under static system for the total exposure of 96 hrs. Water flea collected from Put-In-Bay, Ohio initially and then cultivate and maintain in the laboratory. Effect were observed after 24, 48, 72 and 96 hrs in the form of TLm (Median tolerance limits). Based on the mortality of daphnia magna by the chemical exposure for 96 hrs, the LC50 was determine to be 5067 mg/l. And after the 24, 48hrs and 72hrs, the LC50 was observed at 8665 mg/l and 6017 mg/l. based on the LC50, chemical was consider as nontoxic and not classified as per the CLP classification criteria.

 

In the third supporting study the effective concentration EC0 and EC50 value of sodium 3-nitrobenzenesulphonate in aquatic invertebrate (Daphnia magna) in a 100 hr study on mortality effect was found to be 1000 mg/L and 2335 mg/L respectively. Thus, considering the CLP Criteria for aquatic classification of the substance, it is concluded that the test chemical does not exhibit short term toxicity to aquatic invertebrate (Daphnia magna).

Based on the experimental studies from various sources, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

Long term toxicity to aq. invertebrates:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrates was predicted for test substance. On the basis of no effects observed on a freshwater aquatic invertebrates, the NOEC value for the substance is estimated to be 29119.572 mg/l for daphnia for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to daphnia at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

 

 

Toxicity to algae:

Aim of this study was to assess the short term toxicity of test chemical to aquatic algae and cyanobacteria. Study was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system for the total exposure period of 72 hrs. The solution of white pearl 100 mg/l was prepared by dissolving the substance in OECD growth medium. Test conducted at limit concentration of 100 mg/l. Strain 86.81 SAG were used in the study. 5x10(3) cells/ml were added in 100 ml test vessel. Effects on growth rate were observed for 72 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, inhibitory concentration IC was calculated. The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05. After the exposure of chemical with algae Desmodesmus subspicatus, no inhibition were observed. And the IC0% was determine at 100 mg/l. As no effect were observed at limit dose 100 mg/l thus it was concluded that the EC50 was > 100 mg/. Based on the no effect on the growth of algae, it was concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

 

 

 

Toxicity to microorganisms:

Based on the various experimental data for the test chemical and for structurally and functionally similar read across chemicals study have been reviewed to determine the toxic nature of test chemical on the growth of microorganisms. The studies are as mentioned below:

 

In the first key study for the test chemical used from peer reviewed journal toxicity were measured. Short term toxicity of chemical was studied on the growth of bacteria. The effect concentration (EC50) value of test chemical on microorganism (Bacteria sp.) in a 17 hrs study was observed at a dose concentration of > 10000 mg/L.

 

First experimental study was supported by the second supporting study from peer reviewed journal Study was carried out to evaluate the nature of chemical on the bacterial growth. Study was conducted for 17 hrs in which unadapted biological waste water treatment plants were exposed with chemical. Based on the 17 hrs exposure study of bacteria with the test chemical, the EC50 was determine to be 7000 mg/l on the basis of growth rate inhibition.

 

Aim of this third supporting study was to determine the nature of test chemical on the growth of bacteria. Study was conducted for 17 hrs in which unadapted biological waste water treatment plants were exposed with chemical. Based on the growth rate inhibition of bacteria by the test chemical for 17 hrs, the EC50 was 3800 mg/l. Thus the chemical was not highly toxic to the growth of bacteria.

 

Thus based on the above studies, chemical consider to be nontoxic.