Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 276-038-9 | CAS number: 71808-53-2
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The EC50 daphnia is 468 µg/L based on test material, derived from a 21d-daphnia test according to OECD TG 211. The EC50 is 465 µg/L converted to active ingredient content.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 465 µg/L
Additional information
For Quaternary ammonium compounds, C12-18-alkylbis(hydroxyethyl)methyl as registered, no studies on the acute toxicity to aquatic invertebrates are available. The 21d-EC50 determined in a long term OECD 211 test (reliability 1) is used as a worst case key value for the short term toxicity to aquatic invertebrates. Furthermore, read-across is used as supporting information for this endpoint. The two supporting studies (OECD 202) are performed with quaternary ammonium compound C12 and quaternary ammonium compound C14 (both reliability 4, because no GLP, no certificates of analysis of the technical products were available, nominal concentrations based on test material).
The two read-across studies were conducted in a period when no reliable specific method of analyses was available. The concentrations were therefore not analytically verified. The substance is known for its poor water solubility and the strong tendency to adsorb onto the walls of test vessels. Therefore it was decided to perform a number of higher tier chronic invertebrate studies with analytical monitoring of the test concentrations. These higher tier tests were performed in river water to allow a PECaquatic,bulk/PNECaquatic,bulk approach for the environmental risk assessment. The long term 21d-EC50 for parental mortality determined in the OECD211 test with river water (reliability 1) was used as the key value for acute toxicity for daphnia in the chemical safety assessment.
The 21d-EC50 of 468 ug/l was determined according to OECD TG 211.The study was assigned reliability 1 as all validation criteria were fulfilled (GLP,complete identification of test substance, including chemical analyses). The effects are expressed as nominal values because the tests were performed with river water as they are intended to be used in an evaluation of the environmental risks based on the bulk approach. Further experimental details of the key study are described in the section on long term toxicity to aquatic invertebrates.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
