Residues (petroleum), light vacuum

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: RELIABLE SUMMARY REPORT

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

review article or handbook

Title:

ROBUST SUMMARY OF INFORMATION HEAVY FUEL OILS

Author:

AMERICAN PETROLEUM INSTITUTE

Year:

2003

Bibliographic source:

ROBUST SUMMARY OF INFORMATION HEAVY FUEL OILS PG 26-27

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Fuel oil, residual

EC Number:

270-675-6

EC Name:

Fuel oil, residual

Cas Number:

68476-33-5

IUPAC Name:

68476-33-5

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

not specified

Water media type:

not specified

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

>= 3 - <= 10 mg/L

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

>= 100 - <= 300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Based on nominal loading rates, ranges within which lie 72-hr EL50 (biomass) and 72-hr EL50 (growth rate) were 3-10 mg/l and 100-300 mg/l, respectively. 72- hr NOEL (biomass) = 1mg/l. 72 h Mean Cell Conc.

Nominal Conc.       72 h %       72h Mean Cell Conc

(mg/l)                     Inhibition       (x106 cells/ml)

Control                     n/a              0.12

1.0                            22              0.093

3.0                            19              0.097

10                            46              0.065

30                            58              0.05

100                            44              0.067

300                            77              0.027

1000                            72              0.033

Difference between EbL50 and ErL50 was due to an initial lag followed by recovery at loading rates between 3 and 100 mg/l. The initial lag affected the 72-hr EbL50 and not the 72-hr ErL50. Total peak area of the dissolved components ranged from 1 x 10 ^8 at loading rate of 1mg/l to 16-20 x108at 1000 mg/l. Peak profile was different at different loading rates but peak profile for new and old media was similar. Mean reduction in total peak area was 44% during the test (range 20 -67%). Peak profiles for the WAFs differed significantly from profile of light fuel oil in dichloromethane. There was a maximum pH change of 1.1 which was greater than the target of 1. This was a result of the growth of the cultures and could not be avoided.