Registration Dossier
Registration Dossier
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EC number: 910-404-7 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: It is not clear whether sulfide concentrations were measured or not.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Comparison of the Toxicity Thresholds of Water Pollutants to Bacteria, Algae, and Protozoa in the Cell Multiplication Inhibition Test.
- Author:
- Bringmann G, Kühn R
- Year:
- 1 980
- Bibliographic source:
- Water research 14/3: 231-241
- Reference Type:
- publication
- Title:
- Testing of substances for their toxicity threshold: Model organisms Microcystis (Diplocystis) aeruginosa and Scenedesmus quadricauda.
- Author:
- Bringmann G, Kühn R
- Year:
- 1 978
- Bibliographic source:
- Mitteilungen der Internationalen Verein für Limnologie 21: 275-284
- Reference Type:
- publication
- Title:
- Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Bakterien (Pseudomonas putida) und Grünalgen (Scenedesmus quadricauda).
- Author:
- Bringmann G, Kühn R
- Year:
- 1 977
- Bibliographic source:
- Zeitschrift für Wasser- und Abwasser-Forschung 3: 87-98
- Reference Type:
- publication
- Title:
- Grenzwerte der Schadwirkung wassergefährender Stoffe gegen Blaualgen (Microcystis aeruginosa) und Grünalgen (Scenedesmus quadricauda) im Zellvermehrungs-hemmtest.
- Author:
- Bringmann G, Kühn R
- Year:
- 1 978
- Bibliographic source:
- Vom Wasser 50: 45-60
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1313-84-4
- Cas Number:
- 1313-84-4
- IUPAC Name:
- 1313-84-4
- Reference substance name:
- sodium sulfide nonahydrate
- IUPAC Name:
- sodium sulfide nonahydrate
- Details on test material:
- - Name of test material (as cited in study report): Na2S.9H2O
Constituent 1
Constituent 2
Test organisms
- Test organisms (species):
- Scenedesmus quadricauda
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 8 d
Test conditions
- Details on test conditions:
- - Dilution series were made of a toxicant solution and distilled water. Macro- and microelements were added as follows: 1 mL of macronutrient solution per 10 mL test medium - 0.4 µL of micronutrient solution per 10 mL test medium. Macronutrient solution contains sodium nitrate, dipotassium hydrogen phosphate, magnesium sulphate, calcium chloride, sodium metasilicate, sodium carbonate, citric acid, iron citrate and cyclohexane diaminetetraacetic acid. Micronutrient solution contains boric acid, manganese chloride, zinc sulphate, copper sulphate, sodium molybdate and cobalt chloride. Initial pH of test medium = 7.0.
- Three replica per test concentration
- Temperature = 27°C
- Growth inhibition was determined through measurement of extinction of monochromatic radiation at 578 nm
Results and discussion
Effect concentrations
- Duration:
- 8 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 44 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- other: S2-
- Basis for effect:
- other: Growth inhibition (measurement of extinction of monochromatic radiation at 578 nm).
Applicant's summary and conclusion
- Conclusions:
- In this study, a 8-d LOEC of 44 mg S2-/L was determined for the unicellular green alga Scenedesmus quadricauda.
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