Reaction mass of disodium sulphide and sodium hydrogensulphide

Long-term toxicity to fish

Administrative data

Endpoint:

fish short-term toxicity test on embryo and sac-fry stages

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Confounding effects of hypoxia. Control survival in tests under normoxic conditions was too low in most tests. Highly variable stocking density. No replicates in most tests.

Data source

Reference

Reference Type:

publication

Title:

Effect of hydrogen sulfide on northern pike eggs and sac fry.

Author:

Adelman IR, Smith LL Jr

Year:

1970

Bibliographic source:

Transactions of the American Fisheries Society 99: 501-509

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)

Principles of method if other than guideline:

In this study, northern pike eggs of 24 up to 144 h after fertilization were exposed to a control and a series of sulfide concentrations for 96 h up to 17 days under either hypoxic or normoxic conditions.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen sulphide

EC Number:

231-977-3

EC Name:

Hydrogen sulphide

Cas Number:

7783-06-4

Constituent 2

Reference substance name:

hydrogen sulfide

IUPAC Name:

hydrogen sulfide

Details on test material:

H2S

Test organisms

Test organisms (species):

Esox lucius

Details on test organisms:

Northern pike eggs

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 d

Remarks on exposure duration:

17 days = maximum test duration

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.022 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

H2S

Basis for effect:

mortality

Remarks on result:

other: Lowest LC50, 96-h test, eggs of 60 h after fertilization, hypoxia

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.058 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

H2S

Basis for effect:

mortality

Remarks on result:

other: Highest LC50, 96-h test, eggs of 24 h after fertilization, hypoxia

Applicant's summary and conclusion

Conclusions:

In this study, northern pike eggs (Esox lucius) of 24 up to 144 h after fertilization were exposed to a control and a series of sulfide concentrations for 96 h up to 17 days under either hypoxic or normoxic conditions. The LC50 values varied from 0.022 (96 h exposure, eggs of 60 h after fertilization, hypoxia) to 0.058 mg H2S/L (96 h exposure, eggs of 24 h after fertilization, hypoxia). All LC50 values (whether obtained under normoxic or hypoxic conditions) were very similar.