N-acetylsulphanilyl chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the OECD test guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test concentrations selected for the study were 0, 0.9, 2, 5, 15, 25, 50, 75 and 100 mg/l (nominal concentrations), respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Length at study initiation (length definition, mean, range and SD): average 2.3 cm
- Weight at study initiation (mean and range, SD): average 0.203 g
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 16 hour light and 8 hour darkness
- Diet : Standard brand feed, once daily
- Acclimatization: seven days
- Feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

29°C

pH:

7.0

Dissolved oxygen:

7.5 mg/l

Nominal and measured concentrations:

Nominal concentrations: 0, 0.9, 2, 5, 15, 25, 50, 75 and 100 mg/l (nominal concentrations)

Details on test conditions:

TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2 - < 5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality and symptoms:

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	0	0	0	0
0.9	8	0	0	0	0
2	8	0	0	0	0
5	8	8	-	-	-
15	8	8	-	-	-
25	8	8	-	-	-
50	8	8	-	-	-
75	8	8	-	-	-
100	8	8	-	-	-

 

Visible Symptoms: No visible symptom was observed. Fishes were moving slowly.

Nominal conc. (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
0.9	8	NS8	NS8	NS8	NS8
2	8	NS8	NS8	NS8	NS8
5	8	-	-	-	-
15	8	-	-	-	-
25	8	-	-	-	-
50	8	-	-	-	-
75	8	-	-	-	-
100	8	-	-	-	-

 

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 

pH and oxygen content

Nominal conc. (mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	7.6	6.8	6.8	6.7
0.9	5.6	6.3	6.2	6.3
2	5.2	5.6	5.4	5.3

 

Nominal conc. (mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	7.9	7.5	6.9	6.7
0.9	7.7	7.3	6.3	6.1
2	7.6	7.2	6.2	6.2

 

Test temperature

Nominal conc. (mg/l)	Temperature (°C) after
	24h	48h	72h	96h
0 (control)	29	29	29	29
0.9	29	29	29	29
2	29	29	29	29

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, the median lethal concentrations [LC50 (96 h)] for test chemical on Danio rerio (Zebra Fish) was determined to be in the range of > 2 to < 5 mg/L.

Executive summary:

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.203 g and average length of 2.3 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7 to 8, water temperature 29°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance showed 100% mortalities within 24 hours of the start of the experiment. Thus, on the basis of this, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.9, 2, 5, 15, 25, 50, 75 and 100 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 29°C, pH 7.0, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control vessel. Test fishes were found to be freely swimming in the bowl aquaria but mortalities were observed in the test vessels during the study. On the basis of effect of test chemical on mortality of the test organism, the LC0, median lethal concentration (LC50 (96 h)) and LC100 value was determined to be 2 mg/l, > 2 to < 5 mg/L and 5 mg/l, respectively. Thus, based on the LC50 value, test chemical can be considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, test chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP classification criteria.

Description of key information

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical (Experimental study report, 2013). The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.203 g and average length of 2.3 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7 to 8, water temperature 29°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance showed 100% mortalities within 24 hours of the start of the experiment. Thus, on the basis of this, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.9, 2, 5, 15, 25, 50, 75 and 100 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 29°C, pH 7.0, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control vessel. Test fishes were found to be freely swimming in the bowl aquaria but mortalities were observed in the test vessels during the study. On the basis of effect of test chemical on mortality of the test organism, the LC0, median lethal concentration (LC50 (96 h)) and LC100 value was determined to be 2 mg/l, > 2 to < 5 mg/L and 5 mg/l, respectively. Thus, based on the LC50 value, test chemical can be considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, test chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP classification criteria.

Additional information

Experimental study and various predicted data of the target chemical and supporting weight of evidence study of its structurally similar read across substance were reviewed for the short term toxicity to aquatic invertebrates end point which are summarized as below:

 

In an experimental key study from study report (2013),short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.203 g and average length of 2.3 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7 to 8, water temperature 29°C and under a photoperiod of 16:8 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance showed 100% mortalities within 24 hours of the start of the experiment. Thus, on the basis of this, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.9, 2, 5, 15, 25, 50, 75 and 100 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 29°C, pH 7.0, hardness of water 155.5 mg of CaCO3 and under a photoperiod of 16:8 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. No mortalities were observed in the control vessel. Test fishes were found to be freely swimming in the bowl aquaria but mortalities were observed in the test vessels during the study. On the basis of effect of test chemical on mortality of the test organism, the LC0, median lethal concentration (LC50 (96 h)) and LC100 value was determined to be 2 mg/l, > 2 to < 5 mg/L and 5 mg/l, respectively. Thus, based on the LC50 value, test chemical can be considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, test chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP classification criteria..

 

In a prediction done using EPI Suite ECOSAR version 1.11, theshort-term toxicity of the test chemical to aquatic fish was predicted.On the basis of effect of test chemical observed in a static system on the mortality of the test organism during the 96 hr exposure duration, the lethal effect concentration (LC50) for the test chemical was estimated to be 173.818 mg/l. Thus, based on the LC50 value, test chemical can be considered as non-toxic to aquatic fishes and hence, considered to be ‘not classified’ as per CLP classification criteria.

 

In an another prediction done using Danish QSAR database, the 96 hrs LC50 value of test chemical on the test organism Pimephales promelas (Fathead minnow) was estimated by three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database. On the basis of effect of test chemical on the mortality of the test organism, the 96 hrs LC50 value was estimated to be 61.39 mg/l. Thus, based on the LC50 value, chemical can be considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, test chemical was considered as non-toxic and hence, considered to be 'not classified' as per the CLP classification criteria.

 

For the test chemical, short term fish toxicity was conducted for 96 hrs for assessing the effect of test chemical (authoritative database, 2020). Study was carried out under static conditions. On the basis of the effect of test chemical on mortality of the test fishes, the 96 hrs median lethal concentration (LC50) value was determined to be 77 mg/l. Thus, based on the LC50 value, test chemical can be considered as toxic to aquatic fishes. Since the test chemical is readily biodegradable in water, test chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP classification criteria.

 

On the basis of the above results, it can be concluded that the test chemical was considered as toxic to aquatic fish. Since the test chemical is readily biodegradable in water, test chemical was considered as non-toxic to aquatic fishes at environmental relevant concentrations and hence, considered to be 'not classified' as per the CLP classification criteria.